Photothermal-modulated drug delivery and magnetic relaxation based on collagen/poly(γ-glutamic acid) hydrogel.
Photothermal-modulated drug delivery and magnetic relaxation based on collagen/poly(γ-glutamic acid) hydrogel.
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DOI：
10.2147/ijn.s133078
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发表时间：
2017
影响因子：
8
通讯作者：
Lim YT
中科院分区：
文献类型：
--
作者：
Cho SH;Kim A;Shin W;Heo MB;Noh HJ;Hong KS;Cho JH;Lim YT
可注射和刺激响应型水凝胶在分子成像和药物传递方面引起了人们的关注，因为水凝胶中的诊断或治疗成分可以通过控制各种刺激如酶、温度、pH和光子能量来成像或改变注射部位的微环境。在这项研究中，我们开发了一种新型的可注射和光响应性的复合水凝胶，它由抗癌药物、成像造影剂、生物衍生胶原和多面体阴离子多肽聚(γ-谷氨酸)(γ-PGA)组成。通过引入γ-PGA，将胶原蛋白固有的温度相关相变行为改变为室温下的低粘度溶胶态和体温附近的非流动凝胶状态。此外，还考察了胶原/γ-PGA水凝胶负载吲哚青绿(ICG)后的变温相变行为。利用γ-PGA中丰富的羧酸基团，阳离子荷电的阿霉素(Dox)和疏水性的MnFe2O4磁性纳米粒子也成功地复合到了胶原/γ-PGA水凝胶中。通过近红外光照射胶原/γ-PGA/Dox/ICG/MnFe2O4水凝胶，可以调节Dox的释放动力学和MnFe2O4纳米粒子的磁弛豫。实验结果表明，本研究开发的新型可注射近红外响应型胶原/γ-PGA水凝胶可以作为多种分子成像探针和治疗成分负载后的治疗平台。
Injectable and stimuli-responsive hydrogels have attracted attention in molecular imaging and drug delivery because encapsulated diagnostic or therapeutic components in the hydrogel can be used to image or change the microenvironment of the injection site by controlling various stimuli such as enzymes, temperature, pH, and photonic energy. In this study, we developed a novel injectable and photoresponsive composite hydrogel composed of anticancer drugs, imaging contrast agents, bio-derived collagen, and multifaceted anionic polypeptide, poly (γ-glutamic acid) (γ-PGA). By the introduction of γ-PGA, the intrinsic temperature-dependent phase transition behavior of collagen was modified to a low viscous sol state at room temperature and nonflowing gel state around body temperature. The modified temperature-dependent phase transition behavior of collagen/γ-PGA hydrogels was also evaluated after loading of near-infrared (NIR) fluorophore, indocyanine green (ICG), which could transform absorbed NIR photonic energy into thermal energy. By taking advantage of the abundant carboxylate groups in γ-PGA, cationic-charged doxorubicin (Dox) and hydrophobic MnFe2O4 magnetic nanoparticles were also incorporated successfully into the collagen/γ-PGA hydrogels. By illumination of NIR light on the collagen/γ-PGA/Dox/ICG/MnFe2O4 hydrogels, the release kinetics of Dox and magnetic relaxation of MnFe2O4 nanoparticles could be modulated. The experimental results suggest that the novel injectable and NIR-responsive collagen/γ-PGA hydrogels developed in this study can be used as a theranostic platform after loading of various molecular imaging probes and therapeutic components.