Optical quantal analysis reveals a presynaptic component of LTP at hippocampal Schaffer-associational synapses
Optical quantal analysis reveals a presynaptic component of LTP at hippocampal Schaffer-associational synapses
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DOI：
10.1016/s0896-6273(03)00325-8
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发表时间：
2003-06-05
期刊：
影响因子：
16.2
通讯作者：
Bliss, TVP
中科院分区：
文献类型：
--
作者：
Emptage, NJ;Reid, CA;Bliss, TVP
长时程增强(LTP)的表达机制存在争议，突触前和突触后都有参与的证据。我们使用共聚焦显微镜和钙敏感染料研究了单个可视化突触上的LTP。在LTP诱导之前和之后，在培养的海马片的锥体细胞的远端树突棘中对突触诱发的钙瞬变进行成像。在大多数突触中，从早到诱导后10分钟到至少60分钟，LTP与单个刺激引起突触后呼叫反应的概率增加有关。这些观察结果提供了令人信服的证据，表明在Schaffer-Associative突触中，突触前成分参与了早期LTP的表达。在大多数情况下，LTP的一个新的突触后方面，诱导后脊髓内的商店依赖的诱发的钙瞬变也增加。
The mechanisms by which long-term potentiation (LTP) is expressed are controversial, with evidence for both presynaptic and postsynaptic involvement. We have used confocal microscopy and Ca2+-sensitive dyes to study LTP at individual visualized synapses. Synaptically evoked Ca2+ transients were imaged in distal dendritic spines of pyramidal cells in cultured hippocampal slices, before and after the induction of LTP. At most synapses, from as early as 10 min to at least 60 min after induction, LTP was associated with an increase in the probability of a single stimulus evoking a postsynaptic Call response. These observations provide compelling evidence of a presynaptic component to the expression of early LTP at Schaffer-associational synapses. In most cases, the store-dependent evoked Ca2+ transient in the spine was also increased after induction, a novel postsynaptic aspect of LTP.