Ultraviolet-Visible (UV-Vis) and Fluorescence Spectroscopic Investigation of the Interactions of Ionic Liquids and Catalase
Ultraviolet-Visible (UV-Vis) and Fluorescence Spectroscopic Investigation of the Interactions of Ionic Liquids and Catalase
复制标题
离子液体与过氧化氢酶相互作用的紫外可见 (UV-Vis) 和荧光光谱研究
DOI：
10.1177/0003702816653124
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发表时间：
2016-11-01
影响因子：
3.5
通讯作者：
Hu, Chaobing
中科院分区：
文献类型：
--
作者：
Dong, Xing;Fan, Yunchang;Hu, Chaobing
采用荧光、吸收紫外-可见光谱法研究了9种离子液体对过氧化氢酶活性的抑制作用。在分子水平上研究了乙酰胆碱与过氧化氢酶的相互作用。实验结果表明，il具有抑制过氧化氢酶活性的作用，其抑制能力取决于其化学结构。荧光实验表明，氢键在相互作用过程中起着重要作用。il对过氧化氢酶活性的抑制能力可以简单地用其疏水性和氢键能力来描述。三氟甲烷磺酸盐（TfO−）的抑制效果较弱可能是由于其较大的尺寸，使得其难以通过过氧化氢酶的底物通道到达活性位点。
The inhibitory effects of nine ionic liquids (ILs) on the catalase activity were investigated using fluorescence, absorption ultraviolet-visible spectroscopy. The interactions of ILs and catalase on the molecular level were studied. The experimental results indicated that ILs could inhibit the catalase activity and their inhibitory abilities depended on their chemical structures. Fluorescence experiments showed that hydrogen bonding played an important role in the interaction process. The inhibitory abilities of ILs on catalase activity could be simply described by their hydrophobicity and hydrogen bonding abilities. Unexpected less inhibitory effects of trifluoromethanesulfonate (TfO−) might be ascribed to its larger size, which makes it difficult to go through the substrate channel of catalase to the active site.