Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase.
Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase.
复制标题
甲状腺球蛋白和甲状腺过氧化物酶自身抗体的高灵敏度测定。
DOI：
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发表时间：
1989
影响因子：
9.3
通讯作者：
Bernard Rees Smith
中科院分区：
文献类型：
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作者：
K. Beever;J. Bradbury;D. Phillips;Sandra M. McLachlan;C. Pegg;A. Goral;W. Overbeck;G. Feifel;Bernard Rees Smith
这些高灵敏度的测定是基于甲状腺自身抗体和125 I标记的自身抗原之间的相互作用。将血清样品与标记的甲状腺过氧化物酶（TPO）或甲状腺球蛋白（Tg）一起孵育，以形成抗体标记的抗原复合物。然后通过加入固相蛋白A沉淀复合物。在存在高浓度TPO抗体或Tg抗体的情况下，超过50%的相应标记抗原沉淀，而在不存在自身抗体的情况下仅1-2%沉淀。抗TPO和抗Tg试验的试验间CV分别为3.2%和5.7%。Tg抗体与TPO抗体无交叉反应。结果与基于抗原包被细胞或塑料支持物的其他测定系统的结果高度显著相关，但本文所述的测定方法灵敏度要高得多。对试验数据的Scatchard分析提供了TPO自身抗体（ka约为10（9）L/mol，浓度高达1 g/L）和Tg自身抗体（ka约为4 x 10（10）L/mol，浓度高达1 g/L）的亲和力和血清浓度信息。总的来说，这些检测方法提供了一个灵敏，精确和方便的系统，用于测量和研究甲状腺自身抗体的特性。
These highly sensitive assays are based on the interaction between thyroid autoantibodies and 125I-labeled autoantigens. Serum samples are incubated with labeled thyroid peroxidase (TPO) or thyroglobulin (Tg) to allow the formation of antibody-labeled antigen complexes. The complexes are then precipitated by addition of solid-phase Protein A. In the presence of high concentrations of TPO antibody or Tg antibody, more than 50% of the respective labeled antigen was precipitated, whereas only 1-2% was precipitated in the absence of autoantibody. Interassay CVs were 3.2% and 5.7%, respectively, for the anti-TPO and anti-Tg assays. There was no cross-reactivity between Tg antibody and TPO antibody. Results correlated highly significantly with results from other assay systems based on antigen-coated cells or plastic supports, but the assays described here were considerably more sensitive. Scatchard analysis of the assay data provided information on the affinity and serum concentration of TPO autoantibodies (ka approximately 10(9) L/mol and concentrations up to 1 g/L) and Tg autoantibodies (ka approximately 4 x 10(10) L/mol and concentrations up to 1 g/L). Overall, these assays provide a sensitive, precise, and convenient system for measuring and investigating the properties of thyroid autoantibodies.