Expression of an arctic chickweed dehydrin, CarDHN, enhances tolerance to abiotic stress in tobacco plants
Expression of an arctic chickweed dehydrin, CarDHN, enhances tolerance to abiotic stress in tobacco plants
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DOI：
10.1007/s10725-016-0169-8
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发表时间：
2016-12-01
影响因子：
4.2
通讯作者：
Zhang, Xing-Hai
中科院分区：
文献类型：
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作者：
Hill, William;Jin, Xiao-Lu;Zhang, Xing-Hai
已知脱水蛋白具有多种细胞内保护功能，并增强植物应对非生物胁迫的能力。将来自北极繁缕植物北极卷耳（Cerastium arcticum）（CarDHN）的SK 5型转基因导入烟草植物中。评价了转基因植株的胁迫反应。与未转化的野生型植物相比，表达CarDHN的植物在种子萌发和幼苗生长过程中表现出对盐和渗透胁迫的耐受性提高，并且在-10 ℃冷冻处理中存活。这些CarDHN植株在盐胁迫下也积累了较低水平的游离脯氨酸，并在干旱或盐胁迫下表现出较低的HSP 70和HSP 26转录水平，表明CarDHN可能在细胞或分子水平上对胁迫反应具有多效性。然而，在干旱胁迫下，野生型和表达CarDHN的植物之间没有观察到显著的表型差异。我们的研究表明，北极繁缕的抗盐，渗透和冷冻胁迫的耐受性的具体作用。
Dehydrin proteins are known to serve a wide variety of intracellular protective functions and enhance the capability of plants to deal with abiotic stresses. The gene for a SK5 type dehydrin from the arctic chickweed plant Cerastium arcticum (CarDHN) was introduced into tobacco plants. Stress responses of the transgenic plants were evaluated. Compared to the untransformed wild type plants, the CarDHN-expressing plants exhibited improved tolerance to salt and osmotic stress during seed germination and seedling growth, and survived in -10 A degrees C freezing treatment. These CarDHN plants also accumulated lower levels of free proline under salt stress and exhibited lower transcription levels of HSP70 and HSP26 under drought or salt stress, suggesting a possible pleiotropic effect of CarDHN on stress response at cellular or molecular level. However, no significant phenotypic differences were observed between the wild type and the CarDHN-expressing plants under drought stress. Our study demonstrated the specific role of the arctic chickweed dehydrin in tolerance to salt, osmotic and freezing stress.