The role of the mismatch repair machinery in regulating mitotic and meiotic recombination between diverged sequences in yeast.
The role of the mismatch repair machinery in regulating mitotic and meiotic recombination between diverged sequences in yeast.
复制标题
错配修复机制在调节酵母分歧序列之间有丝分裂和减数分裂重组中的作用。
DOI：
10.1093/genetics/151.4.1299
复制
发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Jinks-Robertson,S
中科院分区：
文献类型：
--
作者：
Chen,W;Jinks-Robertson,S
在酵母中，不相同的重组底物重组效率低于相同的底物，这种抑制作用大部分可归因于错配修复（MMR）机制的作用。在这项研究中，基于内含子的反向重复序列测定系统已被用来直接比较有丝分裂和减数分裂重组率对350 bp的基板之间的变化从82%到100%的序列同一性。重组率数据表明，序列分歧影响有丝分裂和减数分裂重组类似，虽然细微的差异是显而易见的。除了评估重组率作为序列差异的函数外，还通过DNA测序确定涉及94%相同底物的有丝分裂和减数分裂重组事件的终点。终点分析表明，在MMR缺陷型菌株中形成的减数分裂异源双链DNA的程度比野生型菌株中形成的长65%。这些数据与MMR机制干扰重组过程中异源双链体中间体的形成和/或延伸的模型一致。
Nonidentical recombination substrates recombine less efficiently than do identical substrates in yeast, and much of this inhibition can be attributed to action of the mismatch repair (MMR) machinery. In this study an intron-based inverted repeat assay system has been used to directly compare the rates of mitotic and meiotic recombination between pairs of 350-bp substrates varying from 82% to 100% in sequence identity. The recombination rate data indicate that sequence divergence impacts mitotic and meiotic recombination similarly, although subtle differences are evident. In addition to assessing recombination rates as a function of sequence divergence, the endpoints of mitotic and meiotic recombination events involving 94%-identical substrates were determined by DNA sequencing. The endpoint analysis indicates that the extent of meiotic heteroduplex DNA formed in a MMR-defective strain is 65% longer than that formed in a wild-type strain. These data are consistent with a model in which the MMR machinery interferes with the formation and/or extension of heteroduplex intermediates during recombination.