Transplantation of an autologous mesothelial cell sheet prepared from tunica vaginalis prevents post-operative adhesions in a canine model
Transplantation of an autologous mesothelial cell sheet prepared from tunica vaginalis prevents post-operative adhesions in a canine model
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DOI：
10.1089/ten.2006.12.2629
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发表时间：
2006-09-01
影响因子：
--
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
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作者：
Asano, Touko;Takazawa, Ryoji;Okano, Teruo
术后粘连经常引起严重的并发症，如肠梗阻和腹盆疼痛。先前，我们报道了间皮细胞片移植对于防止大鼠模型中的粘连是有效的。我们还提出了一种新的技术，收获自体间皮细胞从图尼卡迷走神经，而无需腹腔内操作。在这项研究中，我们研究了自体间皮细胞片是否可以防止术后腹膜粘连犬粘连模型。从雄性比格犬的图尼卡迷走神经中分离间皮细胞。分离的细胞在纤维蛋白凝胶上培养。我们将这种结构命名为“间皮细胞片。动物接受手术以诱导腹膜粘连形成，然后移植间皮细胞片（片组，n = 4）、纤维蛋白凝胶（纤维蛋白组，n = 4）或不移植材料（假手术组，n = 4）。移植后4周，我们评估粘连形成并对粘连水平进行评分。移植有间皮细胞片的腹壁用间皮覆盖。实验组的总粘连评分明显低于纤维蛋白组和假手术组。这些结果表明，移植自体间皮细胞片可有效预防犬粘连模型中术后粘连形成。我们的间皮细胞片有可能成为外科手术中一种强大的粘连预防材料。
Post-operative adhesions often cause severe complications such as bowel obstruction and abdominopelvic pain. Previously, we reported that transplantation of a mesothelial cell sheet is effective for preventing adhesion in rat model. We also proposed a new technique for harvesting autologous mesothelial cells from tunica vaginalis without intra-abdominal maneuvers. In this study, we examined whether an autologous mesothelial cell sheet can prevent post-operative peritoneal adhesions in a canine adhesion model. Mesothelial cells were isolated from the tunica vaginalis of male beagles. Isolated cells were cultured on fibrin gel. We named this construct the "mesothelial cell sheet.'' Animals underwent surgery to induce peritoneal adhesion formation and were then transplanted with the mesothelial cell sheets (sheet group, n = 4), fibrin gel (fibrin group, n = 4), or no materials (sham group, n = 4). Four weeks after the transplantation, we evaluated adhesion formation and scored adhesion levels. The abdominal wall transplanted with the mesothelial cell sheet was covered with mesothelium. The total adhesion score of the sheet group was significantly lower than that of the fibrin group and the sham group. These results indicated that transplantation of an autologous mesothelial cell sheet is effective for preventing post-operative adhesion formation in the canine adhesion model. Our mesothelial cell sheet has the potential to be a powerful adhesion prophylactic material in surgery.