BAICALIN DECREASES SGK1 EXPRESSION IN THE HIPPOCAMPUS AND REVERSES DEPRESSIVE-LIKE BEHAVIORS INDUCED BY CORTICOSTERONE
BAICALIN DECREASES SGK1 EXPRESSION IN THE HIPPOCAMPUS AND REVERSES DEPRESSIVE-LIKE BEHAVIORS INDUCED BY CORTICOSTERONE
复制标题
黄芩苷降低海马中 SGK1 的表达并逆转皮质酮诱导的抑郁样行为
DOI：
10.1016/j.neuroscience.2015.10.023
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发表时间：
2015-12-17
期刊：
影响因子：
3.3
通讯作者：
Bai, M.
中科院分区：
文献类型：
--
作者：
Li, Y. -C.;Wang, L. -L.;Bai, M.
本研究旨在探讨黄芩苷对外源性皮质酮诱导的抑郁样行为的预防作用及其可能机制。黄芩苷（10和20 mg/kg）给药21 d后，观察大鼠蔗糖偏好试验（SPT）中的蔗糖偏好性和强迫游泳试验（FST）中的不动时间，采用酶联免疫吸附法（ELISA）检测血清皮质酮水平和海马脑源性神经营养因子（BDNF）含量。采用实时定量聚合酶链反应（qPCR）和蛋白质印迹法检测海马mRNA和蛋白质的表达。结果显示，21天的皮质酮注射导致小鼠出现抑郁样行为，包括蔗糖偏好降低和不动持续时间增加。黄芩苷逆转了上述这些行为变化，并恢复了血清皮质酮水平。黄芩苷上调海马糖皮质激素受体（GR）和BDNF的mRNA和蛋白表达，下调血清和糖皮质激素调节激酶1（SGK 1）的表达。此外，黄芩苷显着增加11 β-羟基类固醇脱氢酶-2（11 β-HSD 2）在海马中的蛋白表达。本研究结果证实黄芩苷对皮质酮诱导的小鼠抑郁模型具有抗抑郁样作用，其机制可能与降低血清皮质酮，从而增加海马BDNF有关。(C)2015年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The present study was to investigate whether baicalin can prevent repeated exogenous corticosterone injection-induced depressive-like behaviors and explore its possible mechanisms. After a 21-day treatment with baicalin (10 and 20 mg/kg), sucrose preference in the sucrose preference test (SPT) and immobility time in forced swimming test (FST) were observed, serum corticosterone levels and brain-derived neurotrophic factor (BDNF) contents in the hippocampus were examined by enzyme-linked immunosorbent assay (ELISA). In addition, quantitative real-time polymerase chain reaction (qPCR) and western blot were used to detect the mRNA and protein expression in the hippocampus. The results showed that 21-day cortiscosterone injections caused depressive-like behaviors in mice, including the reduced sucrose preference and increased duration of immobility. Baicalin reversed these behavioral changes described above and restored serum corticosterone levels. Additionally, baicalin up-regulated the mRNA and protein expression of glucocorticoid receptor (GR) and BDNF, accompanied with the down-regulation of serum-and glucocorticoid-regulated kinase 1 (SGK1) in the hippocampus. Moreover, baicalin significantly increased the protein expression of 11 beta-hydroxysteroid dehydrogenase-2 (11 beta-HSD2) in the hippocampus. The present results confirmed the antidepressant-like effects of baicalin in a mice model of depression induced by corticosterone and suggested that its mechanism was possibly involved in reducing serum corticosterone and thereby increasing BDNF in the hippocampus. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.