Cell biology of laryngeal epithelial defenses in health and disease: Further studies
Cell biology of laryngeal epithelial defenses in health and disease: Further studies
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DOI：
10.1177/000348940311200601
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发表时间：
2003-06-01
影响因子：
1.4
通讯作者：
Koufman, JA
中科院分区：
文献类型：
--
作者：
Johnston, N;Bulmer, D;Koufman, JA
这是一个国际合作研究小组的第二份年度报告，该研究小组正在研究喉咽反流（LPR）对喉上皮细胞的影响。临床和实验研究的结果。在LPR患者、对照组和体外模型中分析了碳酸酐酶（CA）、E-钙粘蛋白和MUC基因表达。在LPR患者中，我们发现声带上皮中的CAIII水平降低，后连合上皮中的CAIII水平升高。实验研究证实喉CAIII响应于反流而耗尽。此外，在pH 4.0以上确实会发生细胞损伤。此外，E-钙粘蛋白（跨膜细胞表面分子，在上皮细胞粘附中具有关键功能）不存在于37%的LPR喉标本中。总之，喉上皮缺乏与食管上皮相比的防御能力，这些差异可能导致喉上皮对反流相关损伤的敏感性增加。
This is the second annual report of an international collaborative research group that is examining the cellular impact of laryngopharyngeal reflux (LPR) on laryngeal epithelium. The results of clinical and experimental studies are presented. Carbonic anhydrase (CA), E-cadherin, and MUC gene expression were analyzed in patients with LPR, in controls, and in an in vitro model. In patients with LPR, we found decreased levels of CAIII in vocal fold epithelium and increased levels in posterior commissure epithelium. The experimental studies confirm that laryngeal CAIII is depleted in response to reflux. Also, cell damage does occur well above pH 4.0. In addition, E-cadherin (transmembrane cell surface molecules, which have a key function in epithelial cell adhesion) was not present in 37% of the LPR laryngeal specimens. In conclusion, the laryngeal epithelium lacks defenses comparable to those in esophageal epithelium, and these differences may contribute to the increased susceptibility of laryngeal epithelium to reflux-related injury.