In vitro evaluation of the cytotoxic activity of three epoxy resin-based endodontic sealers
In vitro evaluation of the cytotoxic activity of three epoxy resin-based endodontic sealers
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DOI：
10.4012/dmj.2017-148
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发表时间：
2018-06-01
影响因子：
2.5
通讯作者：
Lo Muzio, Lorenzo
中科院分区：
文献类型：
--
作者：
Troiano, Giuseppe;Perrone, Donatella;Lo Muzio, Lorenzo
本研究的目的是评价AH Plus、Sicura Seal和Top Seal三种环氧树脂根管封闭剂的细胞毒性。采用3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化法和活/死(R)活/细胞毒试验检测MG63成骨样细胞的直接和间接毒性。采用方差分析和Tukey检验对数据进行统计分析，设定5%的显著性水平。与直接和间接细胞活性试验相关的两种结果都表明，所有组的细胞毒作用都明显高于阴性对照组。培养一周后的细胞毒活性显示无直接细胞毒作用，而间接细胞毒作用中等。三种环氧树脂封闭剂(AH Plus、Top Seal和Sicura Seal)在体外对成骨样细胞均表现出中等程度的细胞毒作用。在所分析的封闭剂中没有发现明显的差异。
The aim of the study was to evaluate the cytotoxicity of three epoxy resin-based endodontic sealer, AH Plus, Sicura Seal and Top Seal. Direct and indirect cytotoxicity were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and LIVE/DEAD (R) Viability/Cytotoxicity Assay on MG63 osteoblasts-like cells. Data were statistically analyzed by analysis of variance and Tukey test, setting a significance level of 5%. Both results related to direct and indirect cell viability tests showed that all groups were significantly more cytotoxic than the negative control group. The cytotoxicity activity after one week of culture showed the absence of direct cytotoxicity, while a medium rate of indirect cytotoxicity. All the three epoxy resin-based sealers (AH Plus, Top Seal and Sicura Seal) showed a medium rate of cytotoxicity on osteoblasts-like cells in vitro. No significant difference was found among the sealers analyzed.