Women with preterm birth have a distinct cervicovaginal metabolome.
Women with preterm birth have a distinct cervicovaginal metabolome.
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早产的妇女具有独特的宫颈阴道代谢组。
DOI：
10.1016/j.ajog.2015.03.052
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发表时间：
2015-06
影响因子：
9.8
通讯作者：
Elovitz MA
中科院分区：
文献类型：
--
作者：
Ghartey J;Bastek JA;Brown AG;Anglim L;Elovitz MA
代谢组学具有揭示早产（PTB）发病机制的新途径的潜力。本研究的目的是调查是否宫颈阴道（CV）代谢组在无症状的妇女中与足月分娩的妇女相比有不同。使用从更大的前瞻性队列中收集的CV液进行巢式病例对照研究。在20-24周（V1）和24-28周（V2）期间采集CV液。将自发性肺结核妇女（n=10）与足月分娩妇女（n=10）的代谢组进行比较。使用标准提取溶剂法（Metabolon®，Durham， NC）提取样品并准备分析。采用GC/MS和UPLC MS/MS对样品进行生化分析。方差分析用于检测各组间生化成分的差异。错误发现率被估计为多重比较的结果。在CV液中共鉴定出313种生化物质。与足月出生的人相比，注定患有PTB的人在V1时CV液中有82种生化物质不同，而在V2时有48种生化物质不同（表1）。氨基酸、碳水化合物和肽代谢物在有和没有肺结核的妇女中是不同的。这些数据表明，CV空间在怀孕期间代谢活跃。CV代谢组的变化可以在任何临床症状出现前几周（如果不是几个月）观察到。了解CV代谢组可能有助于揭示PTB的发病机制，并可能提供新的生物标志物来识别最危险的女性。
Metabolomics has the potential to reveal novel pathways involved in the pathogenesis of preterm birth (PTB). The objective of this study was to investigate if the cervico-vaginal (CV) metabolome was different in asymptomatic women destined to have a PTB compared to term birth. A nested case-control study was performed using CV fluid collected from a larger prospective cohort. CV fluid was collected between 20-24 weeks (V1) and 24-28 weeks (V2). The metabolome was compared between women with a spontaneous PTB (n=10) to women who delivered at term (n=10). Samples were extracted and prepared for analysis using a standard extraction solvent method (Metabolon®, Durham, NC). Global biochemical profiles were determined using GC/MS and UPLC MS/MS. ANOVA was used to detect differences in biochemical compounds between the groups. A false discovery rate was estimated to account for multiple comparisons. A total of 313 biochemicals were identified in CV fluid. 82 biochemicals were different in the CV fluid at V1 in those destined to have a PTB compared to term birth while 48 were different at V2 (Table 1). Amino acid, carbohydrate, and peptide metabolites were distinct between women with and without PTB. These data suggest that the CV space is metabolically active during pregnancy. Changes in the CV metabolome may be observed weeks, if not months, prior to any clinical symptoms. Understanding the CV metabolome may hold promise for unraveling the pathogenesis of PTB and may provide novel biomarkers to identify women most at risk.