Sex-Reversed Somatic Cell Cloning in the Mouse
Sex-Reversed Somatic Cell Cloning in the Mouse
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DOI：
10.1262/jrd.09-099e
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发表时间：
2009-10-01
影响因子：
1.8
通讯作者：
Ogura, Atsuo
中科院分区：
文献类型：
--
作者：
Inoue, Kimiko;Ogonuki, Narumi;Ogura, Atsuo
体细胞核移植在基础科学和应用科学领域有许多潜在的应用。然而，它有一个技术上永远无法克服的缺点--《子孙后代的性别》的僵化。在这里，我们报告了一只雌性小鼠在使用未成熟的支持细胞进行核移植后意外出生的案例。在核移植实验中，我们用从新生雄性小鼠身上收集的支持细胞制作了一批27个克隆。其中，一只是雌性幼崽。这个“雄性衍生雌性”克隆人长成了一只正常的成体，并通过与一只小猫的自然交配产生了后代。染色体分析表明，雌性克隆具有39，X核型，表明Y染色体在供体细胞中或在核移植的早期阶段已被删除。这一发现表明，在克隆一只雄性动物后，有可能恢复有性繁殖，这对复活灭绝或濒危物种特别有用。
Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the Sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species.