Influence of Cranberry Proanthocyanidins on Formation of Biofilms by Streptococcus mutans on Saliva-Coated Apatitic Surface and on Dental Caries Development in vivo
Influence of Cranberry Proanthocyanidins on Formation of Biofilms by Streptococcus mutans on Saliva-Coated Apatitic Surface and on Dental Caries Development in vivo
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DOI：
10.1159/000296306
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Vorsa, N.
中科院分区：
文献类型：
--
作者：
Koo, H.;Duarte, S.;Vorsa, N.
蔓越莓粗提物在各种载体中显示出对体外口腔生物膜形成的抑制作用。蔓越莓提取物中原花青素 (PAC) 的存在与针对变形链球菌（例如变形链球菌）特定毒力属性的生物活性有关。 g。抑制葡萄糖基转移酶（Gtf）活性。本研究的目的是确定高度纯化且化学成分明确的蔓越莓 PAC 组分对唾液涂层羟基磷灰石表面上变形链球菌生物膜形成以及 Sprague-Dawley 大鼠龋齿发展的影响。此外，我们还检测了特定 PAC（从低分子量单体和二聚体到高分子量低聚物/聚合物）抑制变形链球菌细胞的 GtfB 活性和糖酵解 pH 值下降的能力，试图鉴定特定的生物活性化合物。在生物膜形成过程中局部应用 PAC (1.5 mg/ml)（暴露 60 秒，每天两次），与用载体对照处理的生物膜相比，产生更少的生物量和更少的不溶性多糖（10% 乙醇，v/v；p < 0.05）。 PAC治疗（每天两次）显着降低了大鼠光滑表面龋齿的发生率，并且与载体对照组相比，龋齿病变的严重程度较轻（p < 0.05）；使用 PAC 治疗的动物的牙沟表面龋齿严重程度也显着降低 (p < 0.05)。此外，特定的 A 型 PAC 低聚物（二聚体到十二聚体；0.1 mg/ml）有效地减少了吸附在唾液涂层羟基磷灰石表面上的 GtfB 合成不溶性葡聚糖，并且还影响了细菌糖酵解。我们的数据表明，蔓越莓 PAC 减少了体外变形链球菌生物膜的形成和体内龋齿的发展，这可能归因于特定生物活性 A 型二聚体和寡聚体的存在。版权所有 (C) 2010 S. Karger AG，巴塞尔
Cranberry crude extracts, in various vehicles, have shown inhibitory effects on the formation of oral biofilms in vitro. The presence of proanthocyanidins (PAC) in cranberry extracts has been linked to biological activities against specific virulence attributes of Streptococcus mutans, e. g. the inhibition of glucosyltransferase (Gtf) activity. The aim of the present study was to determine the influence of a highly purified and chemically defined cranberry PAC fraction on S. mutans biofilm formation on saliva-coated hydroxyapatite surface, and on dental caries development in Sprague-Dawley rats. In addition, we examined the ability of specific PAC (ranging from low-molecular-weight monomers and dimers to highmolecular-weight oligomers/polymers) to inhibit GtfB activity and glycolytic pH drop by S. mutans cells, in an attempt to identify specific bioactive compounds. Topical applications (60-second exposure, twice daily) with PAC (1.5 mg/ml) during biofilm formation resulted in less biomass and fewer insoluble polysaccharides than the biofilms treated with vehicle control had (10% ethanol, v/v; p < 0.05). The incidence of smooth-surface caries in rats was significantly reduced by PAC treatment (twice daily), and resulted in less severe carious lesions compared to the vehicle control group (p < 0.05); the animals treated with PAC also showed significantly less caries severity on sulcal surfaces (p < 0.05). Furthermore, specific A-type PAC oligomers (dimers to dodecamers; 0.1 mg/ml) effectively diminished the synthesis of insoluble glucans by GtfB adsorbed on a saliva-coated hydroxyapatite surface, and also affected bacterial glycolysis. Our data show that cranberry PAC reduced the formation of biofilms by S. mutans in vitro and dental caries development in vivo, which may be attributed to the presence of specific bioactive A-type dimers and oligomers. Copyright (C) 2010 S. Karger AG, Basel