Determination of moxifloxacin in human plasma by liquid chromatography electrospray ionization tandem mass spectrometry
Determination of moxifloxacin in human plasma by liquid chromatography electrospray ionization tandem mass spectrometry
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DOI：
10.1016/s0731-7085(02)00393-x
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发表时间：
2002-11-07
影响因子：
3.4
通讯作者：
Stewart, JT
中科院分区：
文献类型：
--
作者：
Vishwanathan, K;Bartlett, MG;Stewart, JT
莫西沙星是一种先进的8-甲氧基氟喹诺酮类药物，对广谱病原体有活性，包括耐药性肺炎链球菌。建立一种快速、灵敏、高选择性的测定人血浆中利多卡因的方法，对于了解利多卡因口服或静脉给药的药代动力学至关重要。采用Oasis（R）HLB固相萃取（SPE）从血浆中提取利福沙星和内标洛美沙星。建立了一种基于液相色谱电喷雾串联质谱法（LC/ESI-MS/MS）的人血药浓度测定方法。分析物的前体和主要产物离子在具有正离子电喷雾电离（ESI）的三重四极杆质谱仪上以多反应监测（MRM）模式监测。提出了阿西沙星碰撞诱导解离（CID）产物的形成机理。生成的线性校准曲线从1至1000 ng/ml，决定系数大于0.999。日间和日内精密度（% CV）小于11.3%，准确度（%误差）小于10.0%。基于信噪比为3，该方法的检测限（LOD）为50 pg/ml。(C)2002 Elsevier Science B. V.保留所有权利。
Moxifloxacin is an advanced-generation, 8-methoxy fluoroquinolone that is active against a broad spectrum of pathogens, including antibiotic resistant Streptococcus pneumoniae. Development of a rapid, sensitive and selective method for the determination of moxifloxacin in human plasma is essential for understanding the pharmacokinetics of the drug when administered orally or intravenously. Solid phase extraction (SPE) using Oasis(R) HLB was used to extract moxifloxacin and the internal standard lomefloxacin from plasma. A method based on liquid chromatography electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed and validated to quantitate moxifloxacin in human plasma. The precursor and major product ion of the analyte was monitored on a triple quadrupole mass spectrometer with positive ion electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. Mechanisms for the formation of collision-induced dissociation (CID) products of moxifloxacin are proposed. Linear calibration curves were generated from I to 1000 ng/ml with coefficients of determination greater than 0.999. The inter-day and intra-day precision (% CV) was less than 11.3% and accuracy (% error) was less than 10.0% for moxifloxacin. The limit of detection (LOD) for the method was 50 pg/ml based on a signal to noise ratio of 3. (C) 2002 Elsevier Science B.V. All rights reserved.