The cellular and subcellular localization of zinc transporter 7 in the mouse spinal cord.
The cellular and subcellular localization of zinc transporter 7 in the mouse spinal cord.
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DOI：
10.14670/hh-23.781
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发表时间：
2008-07
影响因子：
2
通讯作者：
Zhi-hong Chi;Hao Ren;Xin Wang;Ming Rong;Liping Huang;Zhan-You Wang
中科院分区：
文献类型：
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作者：
Zhi-hong Chi;Hao Ren;Xin Wang;Ming Rong;Liping Huang;Zhan-You Wang
本工作旨在研究锌转运蛋白7（ZNT 7，SLC 30 a7）在小鼠脊髓中的细胞和亚细胞定位以及锌离子（Zn ~（2+））的分布。我们的结果表明，ZNT 7免疫反应阳性神经元广泛分布在所有的脊髓节段的灰质Rexed的板层。中央管的室管膜细胞和白色物质中的胶质细胞也显示ZNT 7阳性。ZNT 7免疫反应主要位于脊髓灰质中ZNT 7阳性细胞的核周区。在室管膜细胞中，ZNT 7的免疫反应性被检测到在中央管腔附近的细胞质中。超微结构定位表明，ZNT 7主要存在于膜的高尔基体堆栈。双重免疫荧光研究证实了这一结果。其他细胞内细胞器，包括内质网，线粒体和溶酶体没有ZNT 7免疫染色。脊髓中可螯合的Zn ~（2+）离子主要存在于神经元的末梢而不是灰质中的细胞体中。然而，在室管膜细胞中发现可螯合的Zn 2+离子和ZNT 7的重叠分布。本研究支持的概念，ZNT 7可能功能提供锌离子的新合成的金属蛋白在脊髓神经元和室管膜细胞的分泌途径。
The present work addresses the cellular and subcellular localization of the zinc transporter 7 (ZNT7, SLC30a7) protein and the distribution of zinc ions (Zn2+) in the mouse spinal cord. Our results indicated that the ZNT7 immunoreactive neurons were widely distributed in the Rexed's laminae of the gray matter in all spinal segments examined. The ependyma cells of the central canal and glia cells in the white matter were also shown ZNT7-positive. The ZNT7 immunoreactivity was mainly detected in the perinuclear regions of ZNT7-positive cells in the spinal gray matter. For ependyma cells, the immunoreactivity of ZNT7 was detected in the cytoplasm near the lumina of the central canal. Ultrastructural localization showed that ZNT7 was predominately present in the membrane of the Golgi stacks. The double immunofluorescence studies confirmed this result. Other intracellular organelles including the endoplasmic reticulum, mitochondria and lysosomes were devoid of ZNT7-immunostaining. The chelatable Zn2+ ions in the spinal cord were found predominantly in the terminals of the neuron rather than the cell body in the gray matter. However, overlapping distribution of chelatable Zn2+ ions and ZNT7 was found in the ependyma cells. The present study supports the notion that ZNT7 may function to supply zinc ions to the newly synthesized metalloproteins in the secretory pathway of the spinal neuron and the ependyma cell.