Citrullus lanatus 'sentinel' (watermelon) extract reduces atherosclerosis in LDL receptor-deficient mice.
Citrullus lanatus 'sentinel' (watermelon) extract reduces atherosclerosis in LDL receptor-deficient mice.
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Citrullus lanatus“哨兵”（西瓜）提取物可减少 LDL 受体缺陷小鼠的动脉粥样硬化。
DOI：
10.1016/j.jnutbio.2012.05.011
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Daugherty,Alan
中科院分区：
文献类型：
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作者：
Poduri,Aruna;Rateri,DebraL;Saha,ShubinK;Saha,Sibu;Daugherty,Alan
西瓜(Citrullus lanatus或C.lanatus)含有许多潜在的生物活性物质，其中包括瓜氨酸，它可能会影响动脉粥样硬化。在这项研究中，我们确定了羊肚菌，作为“哨兵”品种的提取物，对高胆固醇血症诱导的小鼠动脉粥样硬化的影响。8周龄雄性低密度脂蛋白受体缺陷小鼠在饮用饱和脂肪饲料的同时，随机给予羊肚菌提取液(2%VOL/VOL；n=10)或碳水化合物混合物(2%VOL/VOL；n=8)作为对照。食用棉铃虫“哨兵”提取物的小鼠血浆中瓜氨酸浓度显著升高。两组患者的收缩压相近。在不影响瘦肉量的情况下，食用羊肚菌‘前哨’提取物可以降低体重和脂肪质量。两组在食物和水的摄入量以及尿量方面没有差异。给予羊肚菌‘哨兵’提取物可降低血浆胆固醇浓度，这归因于降低中低密度脂蛋白胆固醇。服用羊肚菌前哨提取物的小鼠，血浆单核细胞趋化蛋白-1和干扰素-γ的浓度降低，而白细胞介素10的浓度升高。摄取羊肚菌‘前哨’提取物可减少主动脉弓和胸廓区域的动脉粥样硬化。总之，食用羊肚菌‘前哨’提取物可以减少高胆固醇血症小鼠的体重增加，降低血浆胆固醇浓度，改善促炎和抗炎细胞因子的动态平衡，并在不影响收缩压的情况下减缓动脉粥样硬化的发展。
Watermelon (Citrullus lanatus or C. lanatus) has many potentially bioactive compounds including citrulline, which may influence atherosclerosis. In this study, we determined the effects of C. lanatus, provided as an extract of the cultivar ‘sentinel,’ on hypercholesterolemia-induced atherosclerosis in mice. Male low-density lipoprotein receptor-deficient mice at 8 weeks old were given either C. lanatus ‘sentinel’ extract (2% vol/vol; n=10) or a mixture of matching carbohydrates (2% vol/vol; n=8) as the control in drinking water while being fed a saturated fat-enriched diet for 12 weeks ad libitum. Mice consuming C. lanatus ‘sentinel’ extract had significantly increased plasma citrulline concentrations. Systolic blood pressure was comparable between the two groups. Consumption of C. lanatus ‘sentinel’ extract led to lower body weight and fat mass without influencing lean mass. There were no differences in food and water intake and in urine output between the two groups. C. lanatus ‘sentinel’ extract administration decreased plasma cholesterol concentrations that were attributed to reductions of intermediate-/low-density lipoprotein cholesterol. Plasma concentrations of monocyte chemoattractant protein-1 and interferon-gamma were decreased and those of interleukin-10 were increased in mice consuming C. lanatus ‘sentinel’ extract. Intake of C. lanatus ‘sentinel’ extract resulted in reductions of atherosclerosis in both aortic arch and thoracic regions. In conclusion, consumption of C. lanatus ‘sentinel’ extract led to reduced body weight gain, decreased plasma cholesterol concentrations, improved homeostasis of pro- and anti-inflammatory cytokines, and attenuated development of atherosclerosis without affecting systolic blood pressure in hypercholesterolemic mice.