STRUCTURAL-CHANGES IN THE HEMAGGLUTININ WHICH ACCOMPANY EGG ADAPTATION OF AN INFLUENZA-A(H1N1) VIRUS
STRUCTURAL-CHANGES IN THE HEMAGGLUTININ WHICH ACCOMPANY EGG ADAPTATION OF AN INFLUENZA-A(H1N1) VIRUS
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DOI：
10.1016/0042-6822(87)90040-7
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发表时间：
1987-09-01
期刊：
影响因子：
3.7
通讯作者：
SCHILD, GC
中科院分区：
文献类型：
--
作者：
ROBERTSON, JS;BOOTMAN, JS;SCHILD, GC
甲型H1N1流感病毒和乙型流感病毒的人分离株在胚胎鸡蛋中分离和繁殖过程中，其血凝素（HA）常发生抗原变化。在本报告中，我们描述了A（H1N1）病毒HA的分子变化，这伴随着鸡蛋的适应。卵适应变异的HA1氨基酸取代位点位于受体结合位点附近。这表明，卵细胞适应的变异是在改变受体位点特异性的基础上选择的，同时对HA分子的抗原结构产生影响。在这项研究中，至少观察到三组抗原不同的卵适应变异。这些观察结果对在血清流行病学研究和疫苗生产中不加区分地使用鸡蛋适应病毒具有启示意义。
Antigenic changes are often induced in the haemagglutinin (HA) of human isolates of influenza A(H1N1) and B viruses during their isolation and propagation in embryonated hens'' eggs. In this report we describe molecular changes in the HA of an A(H1N1) virus which accompany egg adaptation. The HA1 amino acid substitutions of egg-adapted variants are located in the vicinity of the receptor binding site. This suggests that egg-adapted variants are selected on the basis of altered receptor site specificity with concomitant effects on the antigenic configuration of the HA molecule. In this study, at least three antigenically distinct groups of egg-adapted variants were observed. These observations have implications for the indiscriminate use of egg-adapted viruses in sero-epidemiological studies and vaccine production.