Characterization and quantification of ginsenosides from the root of Panax quinquefolius L. by integrating untargeted metabolites and targeted analysis using UPLC-Triple TOF-MS coupled with UFLC-ESI-MS/MS
Characterization and quantification of ginsenosides from the root of Panax quinquefolius L. by integrating untargeted metabolites and targeted analysis using UPLC-Triple TOF-MS coupled with UFLC-ESI-MS/MS
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DOI：
10.1016/j.foodchem.2022.132466
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发表时间：
2022-02-21
期刊：
影响因子：
8.8
通讯作者：
Wang, Ying-Ping
中科院分区：
文献类型：
--
作者：
Chen, Li-Hua;Zhang, You-Bo;Wang, Ying-Ping
西洋参（Panax quinquefolius L.）(RPQ)甘草是一种重要的功能性食品，富含生物活性成分甘草皂苷。为全面表征不同来源RPQ中的皂苷类成分并评价其质量，首次将UPLC-Triple TOF-MS与UFLC-ESI-MS/MS联用技术应用于非靶向代谢物和靶向分析。在非靶向代谢物分析中，使用UPLC-Triple TOF-MS结合SWATH数据独立策略从RPQ中鉴定出总共225个皂苷。此外，采用快速、灵敏的UFLC-ESI-MS/MS方法分析了14个RPQ样品中39个靶向标记物的含量。此外，化学计量学分析结果表明，根据目标皂苷的含量，美国RPQ与中国RPQ的质量有所区别。这种新开发的方法为丰富人参皂苷数据库的多样性和评价RPQ的质量提供了有力的工具，可以进一步扩展到其他人参产品和功能食品。
The root of Panax quinquefolius L. (RPQ) is considered as an important functional food and rich in bioactive components, ginsenosides. To comprehensively characterize ginsenosides and evaluate the quality of RPQ from different sources, UPLC-Triple TOF-MS coupled with UFLC-ESI-MS/MS was applied to untargeted metabolites and targeted analysis for the first time. In untargeted metabolites analysis, a total of 225 ginsenosides were identified from RPQ using UPLC-Triple TOF-MS combined with SWATH data-independent strategy. Furthermore, the contents of 39 targeted ginsenoside markers in 14 RPQ samples were analyzed by a rapid and sensitive UFLC-ESI-MS/MS method. In addition, the results of chemometric analysis showed the quality of American RPQ was distinguished from that of Chinese RPQ according to the amount of targeted ginsenosides. This newly developed approach provides a powerful tool for enriching the diversity of saponins database and assessing the quality of RPQ, which can be further extended to other ginseng products and functional foods.