Optimization of experimental variables in the dabsyl chloride derivatization of biogenic amines for their determination by RP-HPLC
Optimization of experimental variables in the dabsyl chloride derivatization of biogenic amines for their determination by RP-HPLC
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DOI：
10.1007/bf02490476
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发表时间：
2000-04-01
期刊：
影响因子：
1.7
通讯作者：
Gázquez, D
中科院分区：
文献类型：
--
作者：
Romero, R;Bagur, MG;Gázquez, D
在过去的几年里，特别注意了生物胺与dabsyl氯的柱前衍生化，因为合适的实验条件对该反应是非常重要的。在这项研究中，实验设计（Doehlert设计）被用来优化以下胺的dabsylation中涉及的变量：组胺，酪胺，苯乙胺，色胺，尸胺，腐胺，亚精胺，精胺。形成dabsyl衍生物的最佳实验条件为：试剂浓度1.75 × 10（-3）M; pH 8.2;温度70 ℃;加热时间（t（h））21 min。
In the last few years special attention has been paid to the pre-column derivatization of biogenic amines with dabsyl chloride because proper experimental conditions for this reaction are very important. In this study, an experimental design (Doehlert design) was used to optimize the variables involved in the dabsylation of the following amines: histamine, tyramine, phenylethylamine, tryptamine, cadaverine, putrescine, spermidine, and spermine. The optimum experimental conditions for forming the dabsyl derivatives are: reagent concentration, 1.75.10(-3) M; pH, 8.2; temperature, 70 degrees C; heating time (t(h)), 21 min. Under these conditions good chromatographic repeatability is obtained.