Mycoparasitism illuminated by genome and transcriptome sequencing of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum
Mycoparasitism illuminated by genome and transcriptome sequencing of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum
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DOI：
10.1099/mgen.0.000345
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发表时间：
2020-03
影响因子：
3.9
通讯作者：
Huizhang Zhao;Ting Zhou;Jiatao Xie;Jiasen Cheng;Tao Chen;D. Jiāng;Yanping Fu
中科院分区：
文献类型：
--
作者：
Huizhang Zhao;Ting Zhou;Jiatao Xie;Jiasen Cheng;Tao Chen;D. Jiāng;Yanping Fu
盾壳霉（Coniothyriumminitans）是一种寄生于核盘菌（Sclerotinia sclerotiorum）的真菌。为了进一步了解该菌的寄生性。我们组装并分析了它的基因组，并进行了转录组分析。C.将minitans菌株ZS-1组装成350个支架并具有39.8Mb的大小。共注释了11437个预测基因和蛋白，其中30.8%的blast hits与Pleosporales的另一个成员Paraphaeosphaeria sporulosa编码的蛋白相匹配，Paraphaeosphaeria sporulosa是一种世界性的土传真菌，具有生防能力。菌株ZS-1在与S.分析了0、4和12小时的菌核病。所检测到的表达基因主要涉及细胞壁降解酶、转运蛋白、分泌蛋白和次生代谢产物等。真菌细胞壁降解酶（FCWD）的17个差异表达基因（DEG）在寄生过程中表达上调，只有1个表达下调。主要易化因子超家族的大部分单羧酸转运蛋白基因和所有检测到的ABC转运蛋白，尤其是重金属转运蛋白，均显著上调。在C.预测minitans是具有催化活性的分泌蛋白。在分子功能类别中，富含水解酶活性、肽酶活性和丝氨酸水解酶活性。大多数参与丝氨酸水解酶活性的基因显著上调。基因组分析和全基因组表达研究表明，C。minitans是复杂的，并且广泛的蛋白质被C.才能成功入侵宿主我们的研究为深入了解C. minitans和S. sclerotiorum和鉴定潜在的次级代谢产物。minitans作为生物防治剂的应用。
Coniothyrium minitans is a mycoparasite of the notorious plant pathogen Sclerotinia sclerotiorum. To further understand the parasitism of C. minitans, we assembled and analysed its genome and performed transcriptome analyses. The genome of C. minitans strain ZS-1 was assembled into 350 scaffolds and had a size of 39.8 Mb. A total of 11 437 predicted genes and proteins were annotated, and 30.8 % of the blast hits matched proteins encoded by another member of the Pleosporales, Paraphaeosphaeria sporulosa, a worldwide soilborne fungus with biocontrol ability. The transcriptome of strain ZS-1 during the early interaction with S. sclerotiorum at 0, 4 and 12 h was analysed. The detected expressed genes were involved in responses to host defenses, including cell-wall-degrading enzymes, transporters, secretory proteins and secondary metabolite productions. Seventeen differentially expressed genes (DEGs) of fungal cell-wall-degrading enzymes (FCWDs) were up-regulated during parasitism, with only one down-regulated. Most of the monocarboxylate transporter genes of the major facilitator superfamily and all the detected ABC transporters, especially the heavy metal transporters, were significantly up-regulated. Approximately 8 % of the 11 437 proteins in C. minitans were predicted to be secretory proteins with catalytic activity. In the molecular function category, hydrolase activity, peptidase activity and serine hydrolase activity were enriched. Most genes involved in serine hydrolase activity were significantly up-regulated. This genomic analysis and genome-wide expression study demonstrates that the mycoparasitism process of C. minitans is complex and a broad range of proteins are deployed by C. minitans to successfully invade its host. Our study provides insights into the mechanisms of the mycoparasitism between C. minitans and S. sclerotiorum and identifies potential secondary metabolites from C. minitans for application as a biocontrol agent.