Transferring the exudate in the tissue engineering chamber as a trigger to incubate large amount adipose tissue in remote area
Transferring the exudate in the tissue engineering chamber as a trigger to incubate large amount adipose tissue in remote area
复制标题
将渗出液转移到组织工程室中作为在偏远地区孵化大量脂肪组织的触发器
DOI：
10.1002/term.2580
复制
发表时间：
2018
影响因子：
3.3
通讯作者：
Wang Biao
中科院分区：
文献类型：
--
作者：
Lei Chen;Dong Ziqing;Wan Jinlin;Xiao Xiaolian;Lu Feng;Wang Biao
组织工程室（TEC）是一种可使脂肪瓣体积增加16倍的技术。但硅胶室中的机制仍然未知。渗出液在腔室中的作用是近年来引起人们注意的。我们开发了一种特殊的模型，称为液体引流模型（FDM），由背部的传统TEC和腹股沟的成对脂肪瓣组成。然后用硅胶管将背侧TEC的渗出液动态转移到配对的腹股沟脂肪瓣中的一个，另一个腹股沟脂肪瓣用假管作为对照。第4周时，引流组的容量达到8.7 ± 2.3 ml，为原始容量的576 ± 152%，而对照组的增长率仅为130 ± 39%。TEC模型组和引流组脂肪瓣体积变化和组织学改变相似。TEC中的渗出液是含有细胞因子以及细胞的异质混合物。有趣的是，TEC模型中转移的渗出液维持了远程孵育大量脂肪组织的能力。
Tissue engineering chamber (TEC) is a technique that could incubate up to 16 folds volume increase of a fat flap. But the mechanism in the silicone chamber was still unknown. The function of exudate in the chamber was noticed recently. We developed a special model called fluid drainage model (FDM) that consisted of a traditional TEC on the back and paired fat flaps without a chamber in the groins. Then we used a silicon tube to dynamically transfer the exudate from dorsal TEC to one of the paired inguinal fat flap and the other inguinal fat flap with a sham tube was set as control. At Week 4, the volume of drainage group reached 8.7 ± 2.3 ml, 576 ± 152% to its original volume whereas the growth ratio of control group was only 130 ± 39%. Similar volume change and histological change were observed within fat flap from TEC model and drainage group. The exudate in the TEC is a heterogeneous cocktail contains cytokines as well as cells. Intriguingly, transferred exudate in the TEC model sustain the ability to incubate large amount of adipose tissue remotely.