Nematicidal effects of the entomopathogenic bacteria Bacillus thuringiensis in soil
Nematicidal effects of the entomopathogenic bacteria Bacillus thuringiensis in soil
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DOI：
10.1078/0031-4056-00160
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发表时间：
2002
期刊：
影响因子：
2.3
通讯作者：
I. N. Mozgovaya;B. Byzov;N. Ryabchenko;N. D. Romanenko;D. Zvyagintsev
中科院分区：
文献类型：
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作者：
I. N. Mozgovaya;B. Byzov;N. Ryabchenko;N. D. Romanenko;D. Zvyagintsev
昆虫病原细菌B的五个菌株杀线虫效果的总结。对苏云金杆菌在体外和种植大麦的土壤中进行了抗线虫的研究。杀线虫活性各不相同的菌株，并与无论是细菌的培养液，细胞沉淀物或高压灭菌的文化。这表明产生了不同的杀线虫因子。在体外试验中，细菌在第2-4天导致高达80%的线虫死亡率。结果发现，除已知的耐热β-外毒素外，B.苏云金杆菌可以产生具有杀线虫活性的热不稳定因子。当大麦种子或土壤用活菌B处理时。苏云金杆菌感染后，细菌孢子萌发，营养细胞迁移到根区并定殖在植物的根表面。无论治疗类型如何，应用B。与对照相比，苏云金杆菌使线虫的繁殖减少了1.5至3倍。未发现针对线虫的单独营养组的特异性杀线虫活性。介绍了B.苏云金杆菌降低了大麦植物的根长和根生物量。
Summary Nematicidal effects of five strains of the entomopathogenic bacterium B. thuringiensis were studied in vitro against nematodes extracted from a soddy-podzolic soil and in situ in the soil planted with barley. Nematicidal activity varied among the strains and was associated with either the culture fluid of the bacteria, the cell sediments or the autoclaved cultures. This suggests different nematicidal factors are produced. In in vitro tests the bacterium caused up to 80% nematode mortality by days 2–4. It was found that, in addition to the known thermo-stable β-exotoxin, B. thuringiensis can produce thermo-labile factor(s) with nematicidal activity. When barley seeds or soil were treated with living cultures B. thuringiensis , bacterial spores germinated and vegetative cells migrated to the root zone and colonised the root surface of the plants. Irrespective of the treatment types, application of B. thuringiensis reduced a multiplication of nematodes by 1.5 to 3 times as compared to the control. No specific nematicidal activity was found towards separate trophic groups of the nematodes. The application of B. thuringiensis reduced root length and root biomass of the barley plants.