Tetrandrine inhibits signal-induced NF-kappa B activation in rat alveolar macrophages
Tetrandrine inhibits signal-induced NF-kappa B activation in rat alveolar macrophages
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DOI：
10.1006/bbrc.1997.6057
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发表时间：
1997-02-03
影响因子：
3.1
通讯作者：
Demers, LM
中科院分区：
文献类型：
--
作者：
Chen, F;Sun, SC;Demers, LM
粉防己碱是一种从天然中草药中分离出来的双苄基异喹啉生物碱。虽然这种生物碱已被证明具有抗纤维化和抗炎活性，但其作用机制尚不清楚。本研究旨在探讨粉防己碱对肺泡巨噬细胞nf - κ B活化的抑制作用。使用三种不同的刺激来激活这些细胞中的nf - κ B。结果表明，粉防己碱可以抑制LPS、PMA和二氧化硅对NF-kappa B和NF-kappa B依赖性报告基因表达的激活，且呈剂量依赖性。相反，在使用的剂量下，粉防己碱不会干扰这些细胞中Sp-1 DNA结合活性或Sp-1依赖的报告基因表达。Western blot分析表明，粉防己碱对NF-kappa B活化的抑制作用可归因于其抑制信号诱导的I kappa B α降解的能力，I kappa B α是NF-kappa B转录因子的细胞质抑制剂。(C) 1997学术出版社。
Tetrandrine is a bisbenzylisoquinoline alkaloid isolated from a natural Chinese herbal medicine. While this alkaloid has been shown to exhibit antifibrotic and anti-inflammatory activities, its mechanism of action is unknown. The present study was designed to investigate the inhibitory effect of tetrandrine on NF-kappa B activation in the alveolar macrophage. Three different provocative stimuli were used to activate NF-kappa B in these cells. The results indicate that tetrandrine can inhibit the activation of NF-kappa B and NF-kappa B-dependent reporter gene expression by LPS, PMA, and silica in a dose-dependent manner. In contrast, at the doses used, tetrandrine did not interfere with Sp-1 DNA binding activity or Sp-1-dependent reporter gene expression in these cells. Western blot analysis suggests that the inhibitory effect of tetrandrine on NF-kappa B activation can be attributed to its ability to suppress signal-induced degradation of I kappa B alpha, a cytoplasmic inhibitor of the NF-kappa B transcription factor. (C) 1997 Academic Press.