Dynamic transcriptome profiling toward understanding the development of the human embryonic heart during different Carnegie stages
Dynamic transcriptome profiling toward understanding the development of the human embryonic heart during different Carnegie stages
复制标题
动态转录组分析有助于了解人类胚胎心脏在不同卡内基阶段的发育
DOI：
10.1002/1873-3468.13930
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发表时间：
2020-10-12
期刊：
影响因子：
3.5
通讯作者：
Sun，Kun
中科院分区：
文献类型：
--
作者：
Meng，Zhuo;Wang，Jian;Sun，Kun
转录调控参与心脏发育。然而，在卡内基阶段（CS）10-CS 16的人胚胎心脏的转录组尚未阐明。在这里，我们发现从CS10到CS 11人类胚胎心脏的形态和转录组发生了显著变化。在CS 12-CS 14，胚胎心脏经历缺氧到有氧转化。在CS 14-CS 16中，转录组功能与能量代谢、胆固醇调节以及与无机物质相关的过程有关。此外，来自人胚胎干细胞（hESC）的心脏祖细胞的转录组与人胚胎心脏的转录组在CS10最重叠。来源于hESC的心肌细胞在CS 14-CS 16与胚胎心脏相当重叠。总的来说，这些结果为人类胚胎心脏发育的特征提供了一个新的视角。
Transcriptional regulation participates in heart development. However, the transcriptomes of human embryonic hearts during Carnegie stage (CS)10–CS16 have not been elucidated. Here, we found marked changes in the morphology and transcriptome of the human embryonic heart from CS10 to CS11. At CS12–CS14, the embryonic heart undergoes hypoxia‐to‐aerobic transformation. At CS14–CS16, transcriptome functions were related to energy metabolism, regulation of cholesterol, and processes related to inorganic substances. Moreover, the transcriptomes of cardiac progenitor cells derived from human embryonic stem cells (hESCs) most overlapped with those of human embryonic hearts at CS10. Cardiomyocytes derived from hESCs considerably overlapped with embryonic hearts at CS14–CS16. Overall, these results provide a new perspective into the characteristics of human embryonic heart development.