Global gene expression profiles for life stages of the deadly amphibian pathogen Batrachochytrium dendrobatidis
Global gene expression profiles for life stages of the deadly amphibian pathogen Batrachochytrium dendrobatidis
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DOI：
10.1073/pnas.0804173105
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发表时间：
2008-11-04
影响因子：
11.1
通讯作者：
Eisen, Michael B.
中科院分区：
文献类型：
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作者：
Rosenblum, Erica Bree;Stajich, Jason E.;Eisen, Michael B.
世界各地的两栖动物正受到一种新出现的病原体的威胁，壶菌菌（Batrachochytrium dendrobaerum）（Bd）。尽管在Bd被发现后的十年里进行了深入的生态学研究，但对Bd杀死青蛙的机制知之甚少。在这里，我们比较模式的全球基因表达在受控的实验室条件下的两个阶段的生命周期的Bd：自由生活游动孢子和基板嵌入孢子囊。我们发现游动孢子在转录上没有孢子囊复杂。游动孢子中更丰富的几个转录本提供了关于这个能动的生命阶段如何与环境相互作用的线索。在孢子囊中表达水平较高的基因提供了新的假说的分子途径参与代谢活动，鞭毛功能，致病性在Bd。我们强调了一组真菌溶素金属肽酶基因的表达模式，一个基因家族被认为是参与致病性的另一组真菌病原体，同样引起皮肤感染的脊椎动物。最后，我们讨论了固有的挑战，在开发一个分子工具包壶菌，一个基础的真菌谱系分离的巨大的系统发育距离，从其他良好的特点真菌。
Amphibians around the world are being threatened by an emerging pathogen, the chytrid fungus Batrachochytrium dendrobatidis (Bd). Despite intensive ecological study in the decade since Bd was discovered, little is known about the mechanism by which Bd kills frogs. Here, we compare patterns of global gene expression in controlled laboratory conditions for the two phases of the life cycle of Bd: the free-living zoospore and the substrate-em bedded sporangia. We find zoospores to be transcriptionally less complex than sporangia. Several transcripts more abundant in zoospores provide clues about how this motile life stage interacts with its environment. Genes with higher levels of expression in sporangia provide new hypotheses about the molecular pathways involved in metabolic activity, flagellar function, and pathogenicity in Bd. We highlight expression patterns for a group of fungalysin metallopeptidase genes, a gene family thought to be involved in pathogenicity in another group of fungal pathogens that similarly cause cutaneous infection of vertebrates. Finally we discuss the challenges inherent in developing a molecular toolkit for chytrids, a basal fungal lineage separated by vast phylogenetic distance from other well characterized fungi.