Structural and Metabolic Relationships Between Goldfish Brain Glycoproteins Participating in Functional Plasticity of the Central Nervous System
Structural and Metabolic Relationships Between Goldfish Brain Glycoproteins Participating in Functional Plasticity of the Central Nervous System
复制标题
参与中枢神经系统功能可塑性的金鱼脑糖蛋白之间的结构和代谢关系
DOI：
10.1111/j.1471-4159.1983.tb08030.x
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发表时间：
1983
影响因子：
4.7
通讯作者：
V. Shashoua
中科院分区：
文献类型：
--
作者：
R. Schmidt;V. Shashoua
摘要/ Abstract摘要：尾管ymins β和γ（分子量为32,000和26,000道尔顿）是金鱼两种分泌的脑糖蛋白，当动物成功获得新的游泳行为模式时，它们的周转率会特别提高。这两种蛋白与豆豆蛋白A结合相同，可以通过凝集素亲和层析从脑细胞外液和脑细胞浆中分离出来。放射免疫分析数据，使用纯化的125I标记的室管ymin和针对室管ymin β或室管ymin γ的抗血清，显示两种蛋白之间完全交叉反应。Scatchardplot分析表明，抗血清在两种蛋白中识别相同的免疫决定因素。这两种室管素的氨基酸组成相似，用金黄色葡萄球菌蛋白酶进行有限的蛋白水解后可得到几种相同的多肽片段。这些蛋白质能够形成γ - 2、β - γ和β - 2的复合物。存在于金鱼脑细胞外液中的一种蛋白酶促进室管素β蛋白水解为室管素γ。研究发现室管ymin γ在生理上来源于室管ymin β，这表明室管ymin β可能在通过更小的多肽片段巩固新的行为模式过程中发挥其生物学功能。
Abstract: Ependymins β and γ (MW 32,000 and 26,000 daltons) are two secreted goldfish brain glycoproteins that exhibit a specifically enhanced turnover rate when the animals successfully acquire a new pattern of swimming behaviour. Both proteins are bound identically to concanavalin A and can be isolated from brain extracellular fluid and from brain cytoplasm by lectin affinity chromatography. Radioimmunoassay data, using purified 125I‐ labeled ependymins and antisera directed against ependymin β or ependymin γ, show complete cross‐reactivity between the two proteins. It is demonstrated by Scatchardplot analysis that the antisera recognize identical immunological determinants in both proteins. The amino acid composition of the ependymins is similar, and several identical polypeptide fragments are obtained after limited proteolysis with Staphylococcus aureus protease. The proteins are capable of forming complexes of the compositions γ2, βγ, and β2. A protease present in the extracellular fluid of goldfish brain promotes proteolysis of ependymin β to ependymin γ. The finding that ependymin γ is physiologically derived from ependymin β suggests the possibility that ependymin β might exert its biological function during consolidation of new behavioural patterns via smaller polypeptide fragments.