The proteome of normal pancreatic juice.
The proteome of normal pancreatic juice.
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DOI：
10.1097/mpa.0b013e31822862f6
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发表时间：
2012-03
期刊：
影响因子：
2.9
通讯作者：
Schmidt CM
中科院分区：
文献类型：
--
作者：
Doyle CJ;Yancey K;Pitt HA;Wang M;Bemis K;Yip-Schneider MT;Sherman ST;Lillemoe KD;Goggins MD;Schmidt CM
本研究的目的是描述正常胰液的蛋白质组，分析促胰液素对正常蛋白质组的影响，并将这些结果与已发表的胰腺癌患者的数据进行比较。三名无明显胰腺病理改变的患者在内窥镜胰腺造影术中获得了成对的胰液标本(静脉注射促胰液素刺激前后)。采用基于质谱学的蛋白质定量技术对蛋白质进行鉴定和定量。人类RefSeq(NCBI)数据库被用来比较正常患者样本中的数据与来自三名胰腺癌患者的公开数据。在正常胰液中共鉴定出285种蛋白质。有90个有足够的氨基酸序列被鉴定，以高度可信的水平表征该蛋白质。所有90种蛋白质在给予促胰液素前后都存在，但在刺激后相对浓度发生了变化，通常是1-2倍。与已发表的170种胰腺癌蛋白质相比，只有42种蛋白质重叠。正常的胰液中含有多种与多种生物过程相关的蛋白质。分泌素可以改变这些蛋白质的浓度，但不会改变它们的光谱。正常人和胰腺癌患者的胰液蛋白质组有明显差异。
The aims of this study were to characterize the proteome of normal pancreatic juice, to analyze the effect of secretin on the normal proteome, and to compare these results with published data from patients with pancreatic cancer. Paired pancreatic fluid specimens (before and after intravenous secretin stimulation) were obtained during endoscopic pancreatography from three patients without significant pancreatic pathology. Proteins were identified and quantified by mass spectrometry-based protein quantification technology. The human RefSeq (NCBI) database was used to compare the data in normal patient samples with published data from three pancreatic cancer patients. A total of 285 proteins were identified in normal pancreatic juice. Ninety had sufficient amino acid sequences identified to characterize the protein with a high level of confidence. All 90 proteins were present before and after secretin administration but with altered relative concentrations, usually by 1-2 folds, after stimulation. Comparison with 170 published pancreatic cancer proteins yielded an overlap of only 42 proteins. Normal pancreatic juice contains multiple proteins related to many biological processes. Secretin alters the concentration but not the spectrum of these proteins. The pancreatic juice proteome of normal and pancreatic cancer patients differ markedly.