Mcm1 binds replication origins
Mcm1 binds replication origins
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DOI：
10.1074/jbc.m209827200
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发表时间：
2003-02-21
影响因子：
4.8
通讯作者：
Tye, BK
中科院分区：
文献类型：
--
作者：
Chang, VK;Fitch, MJ;Tye, BK
Mcm1是酿酒酵母（Saccharomyces cerevisiae）小染色体高效复制和早期细胞周期基因转录调控所必需的蛋白质。在这项研究中，我们报道了Mcm1是一种丰富的蛋白质，它以点状模式与染色质整体相关。我们发现Mcm1定位于复制起点，在体内染色体复制起点的DNA合成起始中起重要作用。使用纯化的Mcm1蛋白，我们发现Mcm1在自主复制序列的多个位点上协同结合。这些结果表明，Mcm1除了作为复制基因表达的转录因子外，还可能通过直接结合影响复制起点的局部结构。
Mcm1 is an essential protein required for the efficient replication of minichromosomes and the transcriptional regulation of early cell cycle genes in Saccharomyces cerevisiae. In this study, we report that Mcm1 is an abundant protein that associates globally with chromatin in a punctate pattern. We show that Mcm1 is localized at replication origins and plays an important role in the initiation of DNA synthesis at a chromosomal replication origin in vivo. Using purified Mcm1 protein, we show that Mcm1 binds cooperatively to multiple sites at autonomously replicating sequences. These results suggest that, in addition to its role as a transcription factor for the expression of replication genes, Mcm1 may influence the local structure of replication origins by direct binding.