Long-term oral administration pf piceatannol (3,5,3',4'-Tetrahydroxystilbene) attenuates colon tumor growth induced by azoxymethane plus dextran sulfate sodium in C57BL/6J mice
Long-term oral administration pf piceatannol (3,5,3',4'-Tetrahydroxystilbene) attenuates colon tumor growth induced by azoxymethane plus dextran sulfate sodium in C57BL/6J mice
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长期口服白皮杉醇（3,5,3,4-四羟基二苯乙烯）可减弱 C57BL/6J 小鼠中氧化偶氮甲烷加葡聚糖硫酸钠诱导的结肠肿瘤生长
DOI：
10.1080/01635581.2021.1985532
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Y. Kimura
中科院分区：
文献类型：
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作者：
日向須美子;森瑛子;花輪壽彦;小田口浩;Y. Kimura
目的 3,5,3',4'-四羟基芪（白皮杉醇）对氧化偶氮甲烷 (AOM)/葡聚糖硫酸钠 (DSS) 诱导的结肠癌生长以及 IL-1β、IL-6、肿瘤坏死因子-α（细胞因子）、MCP-1、血管内皮生长因子和 PD-1 结肠水平变化的影响 方法AOM（10mg/kg，腹腔注射）在第0天诱导结直肠癌发生。第3天，给小鼠提供含有1.5%（w/v）DSSad的水，持续3天，并将该3天的饮用方案重复两次。将白皮杉醇（5 和 12.5 mg/kg，每天两次）口服给予小鼠 7、7、7 和 6 天，然后停药 14、15 和 16 天。通过各自的 ELISA 试剂盒测量细胞因子、趋化因子和 PD-1 结肠水平。结果在给予白皮杉醇的小鼠中(12.5 mg/kg)，肿瘤 数量、肿瘤面积和 Ki-67 阳性细胞数分别减少 30.1%、57.2% 和 89.1%，结肠 MCP-1 和 PD-1 水平分别减少 43.8% 和 70.9%，COX-2 阳性细胞数减少 60.2%。 结论 白皮杉醇对 AOM/DSS 诱导的结肠肿瘤生长的抑制作用似乎是 通过下调肿瘤微环境中 COX-2 的表达，与结肠 MCP-1 和 PD-1 水平降低相关。
AimThe effects of 3,5,3′,4′-tetrahydroxystilbene (piceatannol) on azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colon cancer growth and changes in IL-1β, IL-6, tumor necrosis factor-α (cytokines), MCP-1, vascular endothelial growth factor, and PD-1 colon levels were investigated herein.MethodsAOM (10 mg/kg,i.p.) on day 0 induced colorectal carcinogenesis. On day 3, mice were provided with water containing 1.5% (w/v) DSSad libitumfor 3 day, and this 3-day drinking protocol was repeated twice. Piceatannol (5 and 12.5 mg/kg, twice daily) was orally administered to mice for 7-, 7-, 7-, and 6-day and then discontinued for 14-, 15-, and 16-day.Cytokines, chemokine, and PD-1 colon levels were measured by the respective ELISA kits.ResultsIn mice administered piceatannol (12.5 mg/kg), the tumor number, tumor area, and Ki-67-positive cell numbers decreased by 30.1%, 57.2%, and 89.1%, respectively, colon MCP-1 and PD-1 levels showed reductions of 43.8% and 70.9%, respectively, and COX-2-positive cell numbers declined by 60.2%.ConclusionsThe inhibitory effects of piceatannol on AOM/DSS-induced colon tumor growthappearto be associated with reductions in colon MCP-1 and PD-1 levels through the downregulated expression of COX-2 in the tumor microenvironment.