Ethylene-induced differential gene expression during abscission of citrus leaves.
Ethylene-induced differential gene expression during abscission of citrus leaves.
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DOI：
10.1093/jxb/ern138
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发表时间：
2008
影响因子：
6.9
通讯作者：
Talón M
中科院分区：
文献类型：
--
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
本工作的主要目的是鉴定和分类的基因参与的过程中的叶片脱落的Clementina德Nules（柑橘clementina Hort.Ex Tan。）。一个7 K unigene柑橘cDNA微阵列含有12 K点，用于表征乙烯诱导的叶片脱落过程中的转录组在层状脱落区富集组织和叶柄的脱叶片外植体。在这些条件下，乙烯诱导100%的叶片外植体脱落在72小时，而在空气处理的样品，脱落期开始较晚，需要240小时。在乙烯处理的第一个36小时的基因表达监测表明，在12 672个cDNA微阵列探针，乙烯差异诱导的725个探针分布如下：216（29.8%）的探针在层流区和509（70.2%）的叶柄。差异表达基因的功能MIPS分类和手动注释突出了调节细胞分离的激活和进展的关键过程，从而导致分离。这些包括细胞壁修饰，脂质转运，蛋白质的生物合成和降解，以及信号转导和转录控制途径的差异激活。与叶柄相关的表达数据表明，发生的双重防御策略介导的生化程序，包括清除活性氧，防御和PR基因的激活，和主要基于木质素的生物合成和沉积的物理反应。这项工作确定了新的基因可能参与的发病和发展的叶的drophisation过程中，并提出了不同的，但协调和互补的作用，层状drophisation区和叶柄在drophisation的过程中。
The main objective of this work was to identify and classify genes involved in the process of leaf abscission in Clementina de Nules (Citrus clementina Hort. Ex Tan.). A 7 K unigene citrus cDNA microarray containing 12 K spots was used to characterize the transcriptome of the ethylene-induced abscission process in laminar abscission zone-enriched tissues and the petiole of debladed leaf explants. In these conditions, ethylene induced 100% leaf explant abscission in 72 h while, in air-treated samples, the abscission period started later and took 240 h. Gene expression monitored during the first 36 h of ethylene treatment showed that out of the 12 672 cDNA microarray probes, ethylene differentially induced 725 probes distributed as follows: 216 (29.8%) probes in the laminar abscission zone and 509 (70.2%) in the petiole. Functional MIPS classification and manual annotation of differentially expressed genes highlighted key processes regulating the activation and progress of the cell separation that brings about abscission. These included cell-wall modification, lipid transport, protein biosynthesis and degradation, and differential activation of signal transduction and transcription control pathways. Expression data associated with the petiole indicated the occurrence of a double defensive strategy mediated by the activation of a biochemical programme including scavenging ROS, defence and PR genes, and a physical response mostly based on lignin biosynthesis and deposition. This work identifies new genes probably involved in the onset and development of the leaf abscission process and suggests a different but co-ordinated and complementary role for the laminar abscission zone and the petiole during the process of abscission.
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影响因子：
4.1
作者：
FRY, SC;SMITH, RC;MATTHEWS, KJ
通讯作者：
MATTHEWS, KJ
影响因子：
1.9
作者：
Burns, JK
通讯作者：
Burns, JK
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
7.4
作者：
Desikan, R;Hancock, JT;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
6.4
作者：
GAHAGAN, HE;HOLM, RE;ABELES, FB
通讯作者：
ABELES, FB