Expression of peroxisome proliferator-activated receptors in zebrafish (Danio rerio) depending on gender and developmental stage
Expression of peroxisome proliferator-activated receptors in zebrafish (Danio rerio) depending on gender and developmental stage
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DOI：
10.1007/s00418-004-0737-2
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发表时间：
2005-01-01
影响因子：
2.3
通讯作者：
Cajaraville, MP
中科院分区：
文献类型：
--
作者：
Ibabe, A;Bilbao, E;Cajaraville, MP
过氧化物酶体增殖物激活受体 (PPAR) 是参与哺乳动物胚胎发育和多种组织分化的核激素受体超家族的成员。本研究的目的是调查斑马鱼中三种 PPAR 亚型（PPARα、PPARβ 和 PPARgamma）与性别和发育阶段相关的可能差异表达。为此，通过免疫组织化学方法评估了 7 日龄幼虫、1 个月龄幼虫和 1 岁成虫中 PPAR 的表达。此外，还检查了过氧化物酶体酰基辅酶A氧化酶（AOX）（一种受PPARs调节的基因）的活性，以及过氧化氢酶免疫标记的肝脏过氧化物酶体（V-VP）的体积密度。三种 PPAR 亚型的组织分布或过氧化物酶体 AOX 活性和 V-VP 没有检测到显着的性别相关差异。雌性斑马鱼中 PPARβ 阳性肝细胞的百分比显着高于雄性，表明该亚型在雌性斑马鱼中具有特定的调节作用。这三种 PPAR 亚型在幼虫阶段就已表达，其组织分布模式与成虫相似。对于所有阶段，PPARα 和 PPARβ 的表达水平均高于 PPARβ，并且 PPARβ 免疫标记在幼体阶段比幼虫或成体阶段更强。与 AOX 活性和 V-VP 类似，早期发育阶段肝细胞核免疫标记 PPAR 的百分比高于成人。总之，我们的结果表明 PPAR 表达、其靶基因 AOX 的活性和过氧化物酶体生物发生在斑马鱼中受到发育调节。
Peroxisome proliferator-activated receptors (PPARs) are members of the superfamily of nuclear hormone receptors involved in embryo development and differentiation of several tissues in mammals. The aim of the present study was to investigate the possible differential expression of the three PPAR subtypes (PPARalpha, PPARbeta, and PPARgamma) in relation to gender and developmental stage in zebrafish. For this purpose PPAR expression was assessed by immunohistochemistry in 7-day-old larvae, 1-month-old juveniles, and 1-year-old adults. Additionally, the activity of peroxisomal acyl-CoA oxidase (AOX), a gene regulated by PPARs, and the volume density of catalase-immunolabeled liver peroxisomes (V-VP) was examined. No significant gender-related differences were detected in the tissue distribution of the three PPAR subtypes or in peroxisomal AOX activity and V-VP. The percentage of PPARbeta-positive hepatocytes was significantly higher in females than in males suggesting a specific regulatory role of this subtype in female zebrafish. The three PPAR subtypes were already expressed at the larval stage, with a similar tissue distribution pattern to that found in adults. For all stages, PPARalpha and PPARbeta were expressed at higher levels than PPARbeta, and PPARbeta immunolabeling was stronger in juveniles than in larval or adult stages. The percentages of hepatocyte nuclei immunolabeled for PPARs was higher in early developmental stages than in adults, similarly to AOX activity and V-VP. In conclusion, our results indicate that PPAR expression, the activity of its target gene AOX, and peroxisomal biogenesis are developmentally modulated in zebrafish.