Three-diiensional ex vivo imaging and analysis of intraportal islet transplants
Three-diiensional ex vivo imaging and analysis of intraportal islet transplants
复制标题
门静脉内胰岛移植的三维离体成像与分析
DOI：
10.1111/j.1432-2277.2011.01271.x
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发表时间：
2011
影响因子：
3.1
通讯作者：
Inagaki N
中科院分区：
文献类型：
--
作者：
Fujimoto H;Toyoda K;Okitsu T;Liu X;Mukai E;Zhuang X;Uemoto S;Mochizuki N;Inagaki N
在临床胰岛移植中，由于长期胰岛素依赖率仍然很差，因此需要一种对移植后肝脏中移植胰岛进行详细分析的方法。我们已经建立了一种新的成像技术，适用于分析移植胰岛在肝脏中使用的光学投影断层扫描（OPT）方法。重建肝脏中移植胰岛的三维断层图像。移植的胰岛数和使用OPT观察的移植的胰岛数显示出良好的相关性。采用OPT法比较小鼠同基因和异基因移植模型中移植胰岛的数量。移植同系胰岛的链脲佐菌素（STZ）诱导的糖尿病小鼠的血糖浓度在移植后11天保持正常血糖，移植胰岛的数量在很大程度上得到了保留。 在移植同种异体胰岛的小鼠中，高血糖从移植后7天开始复发，并且移植的胰岛的数量和体积在移植后11天显著减少。 这些结果表明，OPT成像和分析可能是一个有用的工具，定量和空间评价移植的胰岛在肝脏中的细胞水平。
In clinical islet transplantation, because the long‐term insulin‐independence rate is still poor, a method for detailed analysis of the transplanted islets in the liver after transplantation is required. We have established a novel imaging technique suitable for analysis of transplanted islets in liver using an optical projection tomography (OPT) method. A three‐dimensional tomographic image of the transplanted islets in liver was reconstructed. The number of islets transplanted and the number of transplanted islets observed using OPT showed good correlation. The OPT method was used to compare the numbers of transplanted islets in mouse syngeneic and allogeneic transplantation models. Blood glucose concentrations of streptozotocin (STZ)‐induced diabetic mice transplanted with syngeneic islets remained normoglycemic and the number of transplanted islets was largely preserved 11 days after transplantation. In mice transplanted with allogeneic islets, hyperglycemia recurred from 7 days after transplantation and the number and the volume of transplanted islets was significantly reduced 11 days after transplantation. These results indicate that OPT imaging and analysis may be a useful tool to quantitatively and sterically evaluation of transplanted islets in liver at the cellular level.