DDP1, a single-stranded nucleic acid-binding protein of Drosophila, associates with pericentric heterochromatin and is functionally homologous to the yeast Scp160p, which is involved in the control of cell ploidy
DDP1, a single-stranded nucleic acid-binding protein of Drosophila, associates with pericentric heterochromatin and is functionally homologous to the yeast Scp160p, which is involved in the control of cell ploidy
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DOI：
10.1093/emboj/18.13.3820
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发表时间：
1999-07-01
期刊：
影响因子：
11.4
通讯作者：
Azorín, F
中科院分区：
文献类型：
--
作者：
Cortés, A;Huertas, D;Azorín, F
果蝇的着丝粒十二卫星在体外形成改变的DNA结构，其中富含嘌呤的链（G链）形成稳定的折回结构，而互补的C链保持非结构化。在本文中，介绍了 DDP1 的纯化和表征，DDP1 是一种高分子量 (160 kDa) 的单链 DNA 结合蛋白，可特异性结合非结构化十二卫星 C 链。在多线染色体中，DDP1 位于与中心异染色质相关的染色中心，但其分布并不局限于十二卫星序列。 DDP1 还定位于幼虫神经母细胞间期细胞核中的异染色质。在胚胎发育过程中，当异染色质完全组织化时，DDP1 在细胞化后成为细胞核，也与浓缩的有丝分裂染色体相关。除了定位在多线染色体中的染色中心外，还在常染色质臂中与异染色质蛋白 HP1 共定位的多个位点检测到 DDP1。 DDP1 是一种多 KH 结构域蛋白，与参与细胞倍性控制的酵母 Scp160 蛋白同源。 DDP1 的表达补充了酵母中 Delta scp160 的缺失。鉴于 DNA 结构对中心异染色质结构组织的可能贡献，对这些结果进行了讨论。
The centromeric dodeca-satellite of Drosophila forms altered DNA structures in vitro in which its purine-rich strand (G-strand) forms stable fold-back structures, while the complementary C-strand remains unstructured. In this paper, the purification and characterization of DDP1, a single-stranded DNA-binding protein of high molecular mass (160 kDa) that specifically binds the unstructured dodeca-satellite C-strand, is presented. In polytene chromosomes, DDP1 is found located at the chromocentre associated with the pericentric heterochromatin but its distribution is not constrained to the dodeca-satellite sequences. DDP1 also localizes to heterochromatin in interphase nuclei of larval neuroblasts. During embryo development, DDP1 becomes nuclear after cellularization, when heterochromatin is fully organized, being also associated with the condensed mitotic chromosomes. In addition to its localization at the chromocentre, in polytene chromosomes, DDP1 is also detected at several sites in the euchromatic arms co-localizing with the heterochromatin protein HP1. DDP1 is a multi-KH domain protein homologous to the yeast Scp160 protein that is involved in the control of cell ploidy. Expression of DDP1 complements a Delta scp160 deletion in yeast. These results are discussed in view of the possible contribution of DNA structure to the structural organization of pericentric heterochromatin.