A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding
A Rolling Circle Amplification Screen for Polyomaviruses Other than BKPyV in Renal Transplant Recipients Confirms High Prevalence of Urinary JCPyV Shedding
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DOI：
10.1159/000369210
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发表时间：
2015-01-01
期刊：
影响因子：
4.6
通讯作者：
Ganzenmueller, Tina
中科院分区：
文献类型：
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作者：
Kluba, Jeanette;Linnenweber-Held, Silvia;Ganzenmueller, Tina
目的：近年来发现了多种新型人类多瘤病毒（hpyv）。这些或其他未知的嗜肾性hpyv可能通过尿液排出。方法：采用滚动圈扩增（RCA）和定量JCPyV PCR对105例肾移植受者（RTR）的bkpyv阴性尿液进行检测，寻找已知和未知的hpyv。对临床资料进行分析，以确定尿多瘤病毒脱落的危险因素。结果：10%（11/105）尿样的RCA测序显示JCPyV，但未发现其他HPyV序列。采用定量JCPyV PCR检测，24%（25/105）的样本呈阳性。在JCPyV dna阳性的尿液中，RCA检测JCPyV的总灵敏度为44%(11/25)，在JCPyV载量为10万拷贝/ml的样本中，RCA检测JCPyV的总灵敏度为67%（10/15）。尽管在我们的队列中经常检测到尿中有JCPyV脱落，但这与临床危险因素无关。结论：bkpyv阴性、无多瘤病毒相关性肾病的RTR患者，常规尿JCPyV监测诊断价值有限。由于RCA以序列无关的方式工作，因此检测足够数量的新型和已知多瘤病毒是可行的。除BKPyV或JCPyV外，RTR患者尿液中hpyv的高水平脱落不太可能发生。(C) 2015 S. Karger AG，巴塞尔
Objectives: Multiple novel human polyomaviruses (HPyVs) have been discovered in the last few years. These or other, unknown, nephrotropic HPyVs may potentially be shed in urine. Methods: To search for known and unknown HPyVs we investigated BKPyV-negative urine samples from 105 renal transplant recipients (RTR) by rolling circle amplification (RCA) analysis and quantitative JCPyV PCR. Clinical data was analysed to identify risk factors for urinary polyomavirus shedding. Results: In 10% (11/105) of the urine samples RCA with subsequent sequencing revealed JCPyV, but no other HPyV sequences. Using quantitative JCPyV PCR, 24% (25/105) of the samples tested positive. Overall sensitivities of RCA of 44% (11/25) in detecting JCPyV in JCPyV DNA-positive urine and 67% (10/15) for samples with JCPyV loads >10,000 copies/ml can be assumed. Despite frequent detectable urinary shedding of JCPyV in our cohort, this could not be correlated with clinical risk factors. Conclusion: Routine urinary JCPyV monitoring in BKPyV-negative RTR without suspected polyomavirus-associated nephropathy might be of limited diagnostic value. As RCA works in a sequence-independent manner, detection of novel and known polyomaviruses shed in sufficient quantities is feasible. High-level shedding of HPyVs other than BKPyV or JCPyV in the urine of RTR is unlikely to occur. (C) 2015 S. Karger AG, Basel