Genetic analysis of involvement of ETR1 in plant response to salt and osmotic stress
Genetic analysis of involvement of ETR1 in plant response to salt and osmotic stress
复制标题
DOI：
10.1007/s10725-007-9249-0
复制
发表时间：
2008-04
影响因子：
4.2
通讯作者：
Youning Wang;Tao Wang;Kexue Li;Xia Li
中科院分区：
文献类型：
--
作者：
Youning Wang;Tao Wang;Kexue Li;Xia Li
乙烯和乙烯受体乙烯反应1（ETR 1）在植物胁迫反应中的参与已被强调。然而，所涉及的生理过程仍不清楚。本文研究了两个allelesetr 1 - 1和detr 1 - 7突变体在萌发和萌发后幼苗发育过程中对盐胁迫和渗透胁迫的生理响应。etr 1 - 1突变体在萌发和幼苗发育过程中表现出对渗透胁迫（200 mM或更高的甘露醇）和盐胁迫（50 mM或更高的NaCl）的敏感性增加，而etr 1 - 7突变体表现出对严重胁迫（500 mM甘露醇或200 mM NaCl）的耐受性增强。这些结果为乙烯受体ETR 1调节植物对非生物胁迫的反应提供了生理和遗传证据。此外，etr 1 - 1和detr 1 - 7突变体对外源脱落酸（阿坝）的抑制表现出不同的反应。etr 1 - 1突变体在萌发和幼苗发育过程中对阿坝的敏感性高于野生型。与此形成鲜明对比的是，etr 1 - 7突变体对阿坝（>1 μM阿坝）处理的萌发后发育表现出更强的不敏感性，包括根的伸长和子叶的变绿，尽管在试验剂量的阿坝下，萌发没有明显的改变。结果表明，ETR 1调节的胁迫反应可能介导阿坝。
The involvement of ethylene and ethylene receptor Ethylene Response 1 (ETR1) in plant stress responses has been highlighted. However, the physiological processes involved remain unclear. In this study, we have investigated the physiological response of two allelesetr1-1andetr1-7mutants during germination and post-germination seedling development in response to salt and osmotic stress. Theetr1-1mutants showed increased sensitivity to osmotic (200 mM or higher mannitol) and salt stress (50 mM NaCl or higher) during germination and seedling development, whereas theetr1-7mutants displayed enhanced tolerance to the severe stresses (500 mM mannitol or 200 mM NaCl). These results provide physiological and genetic evidence that ethylene receptor ETR1 modulates plant response to abiotic stress. Furthermore, theetr1-1andetr1-7mutants showed different responses to exogenous abscisic acid (ABA) inhibition. Theetr1-1mutants were more sensitive to ABA than the wild type during germination, and young seedling development. In sharp contrast, theetr1-7mutants showed enhanced insensitivity to ABA treatment (>1 μM ABA) in post-germination development including root elongation and greening of cotyledons of the treated seedlings, although the germination was not greatly altered at the tested doses of ABA. The results suggest that ETR1-modulated stress response may mediate ABA.