Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation.
Viability and function of autologous and allogeneic fibroblasts seeded in dermal substitutes after implantation.
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DOI：
10.1111/j.1067-1927.2005.130116ap.x
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
N. Morimoto;Y. Saso;K. Tomihata;Tsuguyoshi Taira;Yoshitake Takahashi;Masayoshi Ohta;Shigehiko Suzuki
中科院分区：
文献类型：
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作者：
N. Morimoto;Y. Saso;K. Tomihata;Tsuguyoshi Taira;Yoshitake Takahashi;Masayoshi Ohta;Shigehiko Suzuki
成纤维细胞种子胶原海绵已被用于皮肤缺损和皮肤溃疡的治疗。然而，成纤维细胞在植入后的生存能力尚不清楚。本研究的目的是研究植入后自体和异体成纤维细胞的生存能力和分布，并阐明哪种类型对伤口愈合更有效。材料与方法取哈特利豚鼠皮肤标本，分离培养自身成纤维细胞。从豚鼠2株皮肤分离成纤维细胞作为同种异体成纤维细胞。在豚鼠背部创面3个全层创面，移植脱细胞胶原海绵、自体成纤维细胞胶原海绵和同种异体成纤维细胞胶原海绵。植入前，用PKH26标记成纤维细胞。植入后1、2、3周处死豚鼠。观察创面的上皮和收缩情况，并在横断面上观察成纤维细胞的活力和分布。结果植入后3周，pkh26标记的自体和异体成纤维细胞仍能存活。自体成纤维细胞胶原蛋白海绵覆盖的创面上皮化速度最快，创面收缩率最小。相比之下，同种异体成纤维细胞覆盖的伤口上皮化最慢，收缩百分比最大。结论胶原海绵中同种异体成纤维细胞在移植区存活，但不能促进创面愈合。
BACKGROUND Fibroblast-seeded collagen sponges have been used for the treatment of skin defects and skin ulcers. However, the viability of the fibroblasts after implantation is still unknown. The objective of this study was to investigate the viability and distribution of autologous and allogeneic fibroblasts after implantation and to clarify which type is more effective for wound healing. MATERIALS AND METHODS Skin samples of Hartley guinea pigs were retrieved and autologous fibroblasts were isolated and cultured. Fibroblasts isolated from the skin of a Strain2 guinea pig were used as allogeneic fibroblasts. Three full-thickness wounds were created on the backs of guinea pigs and an acellular collagen sponge, a collagen sponge seeded with autologous fibroblasts, and a collagen sponge seeded with allogeneic fibroblasts were transplanted. Before implantation, fibroblasts were labeled with PKH26. The guinea pigs were sacrificed 1, 2, and 3 weeks after implantation. The epithelization and contraction of the wounds were assessed, and the viability and distribution of the seeded fibroblasts were observed in cross sections. RESULTS Three weeks after implantation, the PKH26-labeled autologous and allogeneic fibroblasts remained viable. In the wounds covered with the autologous fibroblast-seeded collagen sponge, the epithelization was fastest, and the percent wound contraction was smallest. In contrast, in the wounds covered with allogeneic fibroblasts, the epithelization was slowest and the percent contraction was largest. CONCLUSION The allogeneic fibroblasts seeded in the collagen sponge survived and remained viable on the grafted area, but did not accelerate wound healing.