Anchorage‐dependent cell expansion in fiber‐shaped microcarrier aggregates
Anchorage‐dependent cell expansion in fiber‐shaped microcarrier aggregates
复制标题
纤维状微载体聚集体中贴壁依赖性细胞扩张
DOI：
10.1002/btpr.2755
复制
发表时间：
2018
影响因子：
2.9
通讯作者：
Takeuchi Shoji
中科院分区：
文献类型：
--
作者：
Ikeda Kazuhiro;Takeuchi Shoji
本文描述了一种三维培养系统，用于使用纤维状微载体（MC; Cytodex3）聚集体（称为"MC纤维"）扩增锚定依赖性细胞。纤维包裹细胞、MC聚集体和胶原蛋白，并覆盖有聚左旋赖氨酸膜。纤维的薄结构能够为细胞提供充足的O2和营养物质。使用MC纤维，我们通过连续传代证明了具有高活力的C2C12细胞的有效扩增。因此，我们的培养系统可用于需要大规模细胞扩增的各种应用，例如制药技术，再生医学和培养肉生产。© 2018美国化学工程师学会Biotechnol.进展。，35：e2755，2019.
This article describes a three‐dimensional culture system for the expansion of anchorage‐dependent cells using fiber‐shaped microcarrier (MC; Cytodex3) aggregates, termed “MC fibers.” The fiber encapsulates the cells, the MC aggregates, and collagen and is covered with a poly‐l‐lysine membrane. The thin structure of the fiber enables sufficient supply of O2and nutrients to the cell. Using the MC fiber, we demonstrated the efficient expansion of C2C12 cells with high viability through serial passaging. Therefore, our culture system is useful for various applications where large‐scale cell expansion is required, such as in pharmaceutical technologies, regenerative medicine, and cultured meat production. © 2018 American Institute of Chemical EngineersBiotechnol. Prog., 35: e2755, 2019.