Homeobox gene Hoxa3 is essential for the formation of the carotid body in the mouse embryos
Homeobox gene Hoxa3 is essential for the formation of the carotid body in the mouse embryos
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DOI：
10.1006/dbio.2002.0689
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发表时间：
2002-07-01
影响因子：
2.7
通讯作者：
Chisaka, O
中科院分区：
文献类型：
--
作者：
Kameda, Y;Nishimaki, T;Chisaka, O
同源异型盒基因Hoxa3在第三咽弓和咽囊中强表达。我们发现Hoxa3纯合子无效突变小鼠颈动脉体缺失。在检查的所有晚期突变胚胎（n = 10）中，不存在颈动脉体。颈动脉体原基形成于第三鳃动脉的壁内，发育成颈总动脉和颈内动脉的第一部分。在胚胎第10.5 - 12.5天（E）的野生型同窝仔中观察到第三、第四和第六弓动脉的对称模式。然而，在Hoxa3纯合子突变胚胎中，第三弓动脉在E10.5开始退化，并在E11.5几乎消失。此外，正常位置的颈总动脉分叉，即，在E16.5-E18.5的突变体胚胎中从未检测到。纯合突变体的颈总动脉在其起源后立即分离为颈内动脉和颈外动脉。因此，本研究证明，Hoxa3纯合子突变体中颈动脉体的缺失是由于第三弓动脉发育缺陷，导致颈动脉系统畸形。在胚胎发育过程中，小鼠颈动脉体与交感干的上级颈神经节有密切联系。上级颈神经节，而表现出肥厚的特点，在Hoxa 3纯合子突变体缺乏颈动脉体。(C)2002 Elsevier Science（美国）。
Homeobox gene Hoxa3 is strongly expressed in the third pharyngeal arch and pouch. We found that Hoxa3 homozygous null mutant mice had the lack of the carotid body. In all late-term mutant embryos examined (n = 10), no carotid body was present. The carotid body rudiment is formed in the wall of the third branchial artery, which develops into the common carotid artery and the first part of the internal carotid artery. The symmetrical patterns of the third, fourth, and sixth arch arteries were observed in wild-type littermates at embryonic day (E) 10.5-12.5. In Hoxa3 homozygous mutant embryos, however, the third arch artery began to degenerate at E10.5 and almost disappeared at E11.5. Furthermore, the bifurcation of the common carotid artery at the normal position, i.e., at the upper end of the larynx, was never detected in the mutant embryos at E16.5-E18.5. The common carotid artery of the homozygous mutants was separated into the internal and external carotid arteries immediately after its origin. Thus, the present study evidenced that the absence of the carotid body in Hoxa3 homozygous mutants is due to the defect of development of the third arch artery, resulting in malformation of the carotid artery system. During fetal development, the carotid body of mice is in close association with the superior cervical ganglion of the sympathetic trunk. The superior cervical ganglion rather showed hypertrophic features in Hoxa3 homozygous mutants lacking the carotid body. (C) 2002 Elsevier Science (USA).