Interaction of citrinin and ochratoxin A
Interaction of citrinin and ochratoxin A
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DOI：
10.1002/nt.2620020307
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Friedman, Leonard
中科院分区：
文献类型：
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作者：
Braunberg, Robert C.;Barton, Curtis N.;Friedman, Leonard
霉菌毒素柑橘素和赭曲霉毒素 A 由某些霉菌共同产生，并在许多食品和动物饲料中同时发现。我们使用体外测试来确定这两种霉菌毒素在单独作用和共同作用时是否会产生相同的效果。从年轻成年 Hormel-Hanford 小型猪的肾脏制备的肾皮质立方体在存在或不存在毒素的情况下在 37 摄氏度下培养 1 小时。单独和组合毒素的浓度范围为 10-6 至 10-3 M。将组织孵育、取出、冲洗并重新孵育，以测量四乙铵 (TEA) 或副氨基马尿酸 (PAH) 离子和蛋白质的转运合成，使用3H-亮氨酸。通过最近开发的逻辑函数测试对运输数据进行分析，以确定效果是相加的、协同的还是拮抗的。在混合物中添加效力倍增剂后测试偏差的显着性。测量 TEA 传输的五个实验中的三个的数据表明存在协同效应；对于其他两个，结果与可加性没有显着差异。 PAH 运输也是如此。对于蛋白质合成，一项实验显示出协同作用；对于另一个，非可加性并不显着。没有任何测量结果显示这两种毒素之间存在拮抗作用。与其他几个系统一样，生化效应测试表明，橘霉素和赭曲霉毒素 A 一起给药不会引起一致或强烈的协同反应。
The mycotoxins citrinin and ochratoxin A are produced in common by some molds and have been found together in a number of foods and animal feeds. We used in vitro tests to determine if the same effects are produced by these two mycotoxins when they act both independently and together. Renal cortical cubes prepared from kidneys of young adult Hormel-Hanford miniature swine were cultured in the presence or absence of the toxins for 1 h at 37 degree C. The concentration of the toxins both singly and in combination ranged from 10-6 to 10-3 M. The tissues were incubated, removed, rinsed, and reincubated to measure transport of either tetraethylammonium (TEA) or paraminohippurate (PAH) ions and protein synthesis, using 3H-leucine. The transport data were analyzed by a recently developed logistic function test to ascertain whether the effects were additive, synergistic, or antagonistic. The significance of deviation was tested after a potency multiplier was added to the mixture. Data for three of the five experiments measuring TEA transport indicated a synergistic effect; for the other two, the results were not significantly different from additivity. The same was true for PAH transport. For protein synthesis, one experiment showed synergism; for the other, nonadditivity was not significant. None of the measurements showed antagonism between the two toxins. As with several other systems, tests of biochemical effects showed that administration of citrinin and ochratoxin A together did not elicit either consistent or strong synergistic responses.