Postnatal Catch-up Growth After Fetal Protein Restriction Programs Proliferation of Rat Preadipocytes
Postnatal Catch-up Growth After Fetal Protein Restriction Programs Proliferation of Rat Preadipocytes
复制标题
DOI：
10.1038/oby.2008.417
复制
发表时间：
2008-12-01
期刊：
影响因子：
6.9
通讯作者：
Remacle, Claude A.
中科院分区：
文献类型：
--
作者：
Bol, Vanesa V.;Reusens, Brigitte M.;Remacle, Claude A.
我们研究了大鼠前脂肪细胞的体外增殖和分化，以探讨产前蛋白质限制后的追赶生长是否可能编程脂肪前体细胞，导致脂肪组织质量增加的发展。妊娠大鼠母鼠喂食等热量低蛋白饮食（LP-8%）或对照饮食（C-20%）。在哺乳期间，为了诱导追赶生长，LP组的母鼠饲喂C饲料，窝仔数减少至4只幼仔，而不是8只。从断奶雄性幼仔（28日龄）中分离前脂肪细胞。随时间评估分化和增殖。在前脂肪细胞分化的后期，C或LP培养物的脂质积累没有差异，但LP细胞中瘦素mRNA的表达增强。在培养的早期阶段，在来自LP培养物的脂肪细胞中测量到较高的DNA和蛋白质含量以及较高的增殖速率。此外，在这些细胞中细胞周期蛋白D1的mRNA表达增加，而过氧化物酶体增殖物激活受体γ（PPAR γ）和固醇调节元件结合蛋白（SREBP-1c）的表达在早期阶段显着减少。结果表明，产前暴露于LP，然后快速追赶生长与前脂肪细胞增殖率较高相关。
We studied the in vitro proliferation and differentiation of rat preadipocytes to investigate whether catch-up growth after prenatal protein restriction may program adipose precursor cells leading to development of increased adipose tissue mass. Pregnant rat dams were fed either an isocaloric low-protein diet (LP-8%) or control diet (C-20%). During lactation, in order to induce catch-up growth, dams from LP group were fed with the C diet and litter size was reduced to four pups instead of eight. Preadipocytes were isolated from weanling male pups (28 days of age). Differentiation and proliferation were assessed across time. At late stages of preadipocyte differentiation, no difference was observed in lipid accumulation of C or LP cultures but the mRNA expression of leptin was enhanced in LP cells. At early stages of culture, a higher DNA and protein content accompanied by a higher rate of proliferation was measured in adipocytes from LP cultures. Moreover, the mRNA expression of cyclin D1 was increased in these cells whereas the expression of peroxisome proliferators-activated receptor gamma (PPAR gamma) and steroyl regulatory element binding protein (SREBP-1c) was significantly reduced during early stages. The results suggest that prenatal exposure to a LP followed by rapid catch-up growth is associated with a higher rate for proliferation in preadipocytes.