Serum and ascitic fluid interleukin-17 in spontaneous bacterial peritonitis in Egyptian patients with HCV-related liver cirrhosis
Serum and ascitic fluid interleukin-17 in spontaneous bacterial peritonitis in Egyptian patients with HCV-related liver cirrhosis
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DOI：
10.1016/j.retram.2020.03.001
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发表时间：
2020-11-01
影响因子：
4.1
通讯作者：
Saad, Zienab Mostafa
中科院分区：
文献类型：
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作者：
Keryakos, Hesham Kamal Habeeb;Mohammed, Ahmed Ali;Saad, Zienab Mostafa
背景：自发性细菌性腹膜炎（SBP）是腹水的一种潜在致命并发症。目的：探讨血清和腹水中IL-17 A作为SBP诊断指标的价值及其与肾功能的相关性。方法：采用免疫组织化学方法检测血清和腹水中IL-17 A，并与肾功能进行相关性分析。入选120例丙肝患者，其中80例为HCV诱导的丙肝腹水但未合并SBP，40例为HCV诱导的丙肝腹水合并SBP。结果：肝硬化合并SBP患者血清和腹水中IL-17水平明显高于肝硬化无SBP患者（P < 0.001）;此外，我们发现成功治疗SBP后血清和腹水IL 17水平均显著下降（p < 0.001）。以92 pg/mL为界值，血清IL 17的敏感性和特异性均为100%。结论：IL-17可作为诊断SBP的生物标志物，特别是在培养阴性和非腹水型SBP中，并可用于监测治疗效果。此外，还显示与肝肾功能恶化有关。(C)2020 Elsevier Masson SAS。All rights reserved.
Background: Spontaneous bacterial peritonitis (SBP) is a potentially lethal complication of ascites. The inflammatory response is very intense in case of SBP despite low concentration of bacteria in the ascitic fluid with IL-17A overproduced by intestinal Paneth cells and may have role in host immune defense and inflammatory response.Aims: To study the diagnostic performance of serum and ascitic fluid IL-17A as a marker of SBP and its correlation with renal function.Methods: 120 cirrhotic patients including 80 patients with HCV-induced cirrhotic ascites but not with SBP and 40 patients with HCV-induced cirrhotic ascites with SBP were recruited. Serum and ascitic fluid IL17A were measured before and after treatment.Results: The mean serum and ascitic fluid levels of IL-17 in cirrhotic patients with SBP were significantly higher than in patients with cirrhosis without SBP (p < 0.001). Also, we found significant decline in both serum and ascitic fluid IL17 levels with successful treatment of SBP (p < 0.001). The sensitivity and specificity of serum IL17 was 100 % when using 92 pg/mL as cutoff. Meanwhile, sensitivity and specificity of ascitic fluid IL-17were 100 % when using 132 pg/mL as cutoff.Conclusions: IL-17 could be used as a possible diagnostic biomarker for SBP especially in culture negative and non-neutrocytic SBP and in monitoring therapeutic response. Also, it was shown to be related to hepatic and renal functions deterioration. (C) 2020 Elsevier Masson SAS. All rights reserved.