Biological activity and quantification of suspected allelochemicals from alfalfa plant parts
Biological activity and quantification of suspected allelochemicals from alfalfa plant parts
复制标题
DOI：
10.1046/j.1439-037x.2002.00574.x
复制
发表时间：
2002-08-01
影响因子：
3.5
通讯作者：
Kim, JD
中科院分区：
文献类型：
--
作者：
Chon, SU;Kim, JD
自毒作用限制了紫花苜蓿补种。在紫花苜蓿之后，直到自毒化学物质(S)分解或分散到外部环境中。用紫花苜蓿的叶、茎、根和种子的一系列水提物对同一品种的紫花苜蓿幼苗进行生物测定，以确定其自毒作用。叶提取物的抑制率最高。40g干组织提取液对根、茎和种子的毒性分别是根、茎和种子的15.4、17.5和28.7倍。对9种标准化合物的高效液相色谱分析表明，化感物质的浓度和组成与植物部位有关。在对根生长抑制作用最强的叶提取物中，化感物质的含量最高。在测定的9种酚类化合物中，香豆素、反式肉桂酸和邻香豆酸在10(-3)m处对紫花苜蓿根生长的抑制作用最强。在紫花苜蓿植株体内发现的致病化感物质的种类和数量与生物测定结果高度相关，表明不同紫花苜蓿植株部位的自毒作用存在显著差异。
Autotoxicity restricts reseeding of alfalfa (Medicago sativa L.) after alfalfa until autotoxic chemical(s) breaks down or is dispersed into external environments. A series of aqueous extracts from leaves, stems, roots and seeds of alfalfa 'Vernal' were bioassayed against alfalfa seedlings of the same cultivar to determine their autotoxicity. The highest inhibition was found in the extracts from the leaves. Extracts at 40 g dry tissue l(-1) from alfalfa leaves were 15.4, 17.5 and 28.7 times more toxic to alfalfa root growth than were those from roots, stems and seeds, respectively. A high-performance liquid chromatography (HPLC) analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compounds depended on the plant parts. In leaf extracts that showed the most inhibitory effect on root growth, the highest amounts of allelochemicals were detected. Among nine phenolic compounds assayed for their phytotoxicity on root growth of alfalfa, coumarin, trans -cinnamic acid and o -coumaric acid at 10(-3) m were most inhibitory. The type and amount of causative allelochemicals found in alfalfa plant parts were highly correlated with the results of the bioassay, indicating that the autotoxic effects of alfalfa plant parts significantly differed.