A study of the proliferative activity of the long junctional epithelium using argyrophilic nucleolar organizer region (AgNORs) staining.
A study of the proliferative activity of the long junctional epithelium using argyrophilic nucleolar organizer region (AgNORs) staining.
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DOI：
10.1111/j.1600-0765.1998.tb02203.x
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发表时间：
1998-07
影响因子：
3.5
通讯作者：
T. Uno;S. Hashimoto;M. Shimono
中科院分区：
文献类型：
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作者：
T. Uno;S. Hashimoto;M. Shimono
用核仁组成区嗜银蛋白（AgNORs蛋白）染色法检测大鼠长结合上皮（LJE）的增殖活性。在实验中，通过在上颌磨牙之间插入橡胶片1周来产生LJE。取下橡胶后，测量LJE的长度和AgNORs参数，并进行统计学分析。LJE广泛覆盖暴露根面的根尖侧4周后删除。其长度在4和8周后最长，随后变短。AgNORs在切片上可见不同大小和数量的黑点。4周后，LJE明显具有高增殖潜力，并维持至切除后12周。4-12 wk后，LJE结缔组织界面AgNORs比值约为正常交界上皮的2倍。4 wk后根面侧的增殖活性略有增加。冠侧和根尖侧之间的增殖活性无显著差异。这些结果表明，LJE的增殖活性在供应上皮细胞的结缔组织界面上持续保持在高水平。基底细胞在LJE的结缔组织界面处增殖，直接迁移到根表面或通过根尖部分，最后从上皮表面脱落。
The proliferative activity of the long junctional epithelium (LJE) in rats was examined using stains for argyrophilic proteins of the nucleolar organizer region (AgNORs protein). The LJE was experimentally produced by insertion of a rubber piece between maxillary molars for 1 wk. After removal of the rubber, the length and AgNORs parameters of the LJE were measured and analyzed statistically. The LJE widely covered the apical side of the exposed root surface 4 wk after the removal. Its length was longest after 4 and 8 wk; it became shorter subsequently. The AgNORs were visible as black dots of various sizes and numbers on the sections. A high potential for proliferation was obvious in the LJE after 4 wk and was maintained until 12 wk after the removal. The AgNORs ratio on the connective tissue interface of the LJE was about twice of that of normal junctional epithelium after 4-12 wk. The proliferative activity on the root surface side was slightly increased after 4 wk. There was no significant difference in proliferative activity between the coronal and apical sides. These results suggest that the proliferative activity of the LJE is maintained continuously at a high level on the connective tissue interface supplying the epithelial cells. Basal cells proliferate at the connective tissue interface of the LJE, migrate directly to the root surface or via the apical portion and finally desquamate from the surface of the epithelium.