Identification of an Arabidopsis Plasma Membrane–Located ATP Transporter Important for Anther Development[W]
Identification of an Arabidopsis Plasma Membrane–Located ATP Transporter Important for Anther Development[W]
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拟南芥质膜定位 ATP 转运蛋白对花药发育重要的鉴定[W]
DOI：
10.1105/tpc.111.084574
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发表时间：
1944
期刊：
影响因子：
11.6
通讯作者：
Neuhaus HE
中科院分区：
文献类型：
--
作者：
Rieder B;Neuhaus HE
三磷酸腺苷是植物体内的一种胞外信号分子。然而，将这种化合物输出到质外体的机制的性质仍在争论中。我们确定PM-ANT1蛋白是一种能够介导ATP输出的候选转运蛋白。PM-ANT1加入线粒体载体家族，缺乏细胞器定位所需的N末端氨基酸延伸，并定位于质膜。重组PM-ANT1转运ATP，该基因在成熟的花粉粒中大量表达。人工microRNA(AmiRNA)突变体表现出角果长度缩短，每角果种子数减少，但种子重量增加，而花粉生活力不变。来自amiRNA突变体的花药表现出正常的早期发育，但气孔破裂受到抑制，导致花药开裂受阻。这会导致自花授粉减少，从而降低受精效率。AmRNA花粉粒表现为胞内ATP水平升高，胞外ATP水平下降。后者的作用与重组PM-ANT1的转运特性一致，在植物中支持功能性PM-ANT1驻留在质膜上，并与PM-ANT1的表达模式一致。我们推测PM-ANT1在花粉成熟过程中参与了ATP的输出。ATP输出可能是花药开裂所需的胞外信号，是授粉和自交的一个新的关键因子。
ATP acts as an extracellular signal molecule in plants. However, the nature of the mechanisms that export this compound into the apoplast are under debate. We identified the protein PM-ANT1 as a candidate transporter able to mediate ATP export. PM-ANT1 joins the mitochondrial carrier family, lacks an N-terminal amino acid extension required for organelle localization, and locates to the plasma membrane. Recombinant PM-ANT1 transports ATP, and the gene is substantially expressed in mature pollen grains. Artificial microRNA (amiRNA) mutants show reduced silique length and less seeds per silique but increased seed weight associated with unchanged pollen viability. Anthers from amiRNA mutants exhibited a normal early development, but stomium breakage is inhibited, leading to impaired anther dehiscence. This results in reduced self-pollination and thus decreased fertilization efficiency. amiRNA pollen grains showed increased intracellular ATP levels but decreased extracellular ATP levels. The latter effects are in line with transport properties of recombinant PM-ANT1, supporting in planta that functional PM-ANT1 resides in the plasma membrane and concur with thePM-ANT1expression pattern. We assume that PM-ANT1 contributes to ATP export during pollen maturation. ATP export may serve as an extracellular signal required for anther dehiscence and is a novel factor critical for pollination and autogamy.
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影响因子：
4.4
作者：
Mitra, Srijeet K.;Walters, Benjamin T.;Goshe, Michael B.
通讯作者：
Goshe, Michael B.
分离的完整花椰菜芽质体对 6-磷酸葡萄糖掺入淀粉的表征
影响因子：
7.4
作者：
H.;E. Neuhaus;Cudrun Henrichs;R. Scheibe
通讯作者：
R. Scheibe
影响因子：
--
作者：
M. Stitt;R. Lilley;Richard Gerhardt;H. Heldt
通讯作者：
M. Stitt;R. Lilley;Richard Gerhardt;H. Heldt
植物载体蛋白的 N 端可裂解延伸负责有效插入线粒体内膜。
影响因子：
5.6
作者：
M. Murcha;A. Millar;J. Whelan
通讯作者：
J. Whelan
影响因子：
7.2
作者：
Reinhold, Thomas;Alawady, Ali;Neuhaus, H. Ekkehard
通讯作者：
Neuhaus, H. Ekkehard