IDENTIFICATION OF QUANTITATIVE TRAIT LOCI (QTLS) FOR HEADING DATE AND PLANT HEIGHT IN CULTIVATED RICE (ORYZA-SATIVA L)
IDENTIFICATION OF QUANTITATIVE TRAIT LOCI (QTLS) FOR HEADING DATE AND PLANT HEIGHT IN CULTIVATED RICE (ORYZA-SATIVA L)
复制标题
DOI：
10.1007/bf00220902
复制
发表时间：
1995-07-01
影响因子：
5.4
通讯作者：
PARK, WD
中科院分区：
文献类型：
--
作者：
LI, ZK;PINSON, SRM;PARK, WD
‘莱蒙’和‘特青’都是半矮秆水稻品种，抽穗期相差仅6天。而‘Lemont’与‘特青’杂交，抽穗期和株高均存在超亲分离。通过测试2418 F-4系与113个分布良好的RFLP标记，我们确定和映射的染色体区域，主要负责这种超亲分离。QHd 3a是一个来自Lemont的QTL，它使抽穗提前8天，位于第3号染色体上，距离标记RG 348约3cM。另一个效应较大的QTL QHd 8a位于‘特青’第8染色体上，位于RG 20 ~ RG 1034之间，使抽穗提前7天。沿着一个QTL，QHd 9a的表型效应为3.5天，这些基因组区域共同解释了76.5%的抽穗期观察到的表型方差。4个QTL改变株高从4至7厘米也被定位，这些共同解释48.8%的观察到的株高表型变异。没有一个控制株高的QTL定位在第1染色体上，即半矮秆基因sd-1。所有三个HD位点定位到与PH QTL大致相同的基因组位置，并且在所有情况下，早抽穗的每一天高度都减少约1 cm。HD和一些PH基因座之间的对应关系表明，在这些染色体位置的基因可能有多效性的影响HD和PH。HD的F-1植株中观察到的杂种优势在很大程度上可以解释由显性早熟的确定HD基因座和分布在父母的早抽穗等位基因。然而，在PH QTL之一观察到的超显性可能，至少部分，是负责观察到的杂种优势在PH。
'Lemont' and 'Teqing' are both semidwarf rice varieties that differ in heading date by only 6 days. However, when 'Lemont' and 'Teqing' are crossed there is transgressive segregation for both heading date (HD) and plant height (PH). By testing 2418 F-4 lines with 113 well-distributed RFLP markers, we identified and mapped chromosomal regions that were largely responsible for this transgressive segregation. QHd3a, a QTL from 'Lemont' that gives 8 days earlier heading, was identified on chromosome 3 approximately 3 cM from the marker RG348. Another QTL with a large effect, QHd8a, which gives 7 days earlier heading, was identified on chromosome 8 of 'Teqing' between RG20 and RG1034. Along with a QTL, QHd9a with a phenotypic effect of 3.5 days, these genomic regions collectively explain 76.5% of the observed phenotypic variance in heading date. Four QTLs which altered plant height from 4 to 7 cm were also mapped; these collectively explain 48.8% of the observed phenotypic variation in plant height. None of the QTLs for plant height mapped to chromosome 1, the location of the semidwarf gene sd-1. All three of the HD loci mapped to approximately the same genomic locations as PH QTLs, and in all cases, there was a reduction in height of approximately 1 cm for every day of earlier heading. The correspondence between the HD and some of the PH loci suggests that genes at these chromosome locations may have pleiotropic effects on both HD and PH. The observed heterosis in the F-1 plants for HD can be largely explained by the dominance for earliness of the identified HD loci and distribution of earlier heading alleles in the parents. However, overdominance observed at one of the PH QTL may, at least in part, be responsible for the observed heterosis in PH.