Newcastle disease virus in West Africa: new virulent strains identified in non-commercial farms
Newcastle disease virus in West Africa: new virulent strains identified in non-commercial farms
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DOI：
10.1007/s00705-008-0269-5
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Muller, Claude P.
中科院分区：
文献类型：
--
作者：
Snoeck, Chantal J.;Ducatez, Mariette F.;Muller, Claude P.
对2002年至2007年从尼日尔、尼日利亚、布基纳法索和喀麦隆等国不同禽类中分离的44株纽卡斯尔病毒（NDV）进行了部分F基因序列分析。谱系2病毒与当地使用的LaSota疫苗株在遗传上相同或相似，大多数在商业农场中检出。谱系1、3和4菌株仅零星发现，其来源不太清楚。来自后院农场和活禽市场的21个菌株在谱系5内形成了三个新的集群，暂命名为5f，5g和5h。根据F蛋白裂解位点序列预测所有这些菌株都是毒性的。新的和以前建立的亚系之间的最小遗传距离为9.4%至15.9%，新的亚系之间的最小距离为11.5%至17.3%。它们的高遗传多样性和它们在三个不同的撒哈拉以南国家的存在表明，这些新的亚系代表了西非本土的NDV变体。
Forty-four Newcastle disease virus (NDV) strains, obtained between 2002 and 2007 from different poultry species in Nigeria, Niger, Burkina Faso and Cameroon, were phylogenetically analysed based on partial F sequences. Lineage 2 viruses were genetically identical or similar to the locally used LaSota vaccine strain and were mostly detected in commercial farms. Lineage 1, 3 and 4 strains were only sporadically found, and their origin was less clear. Twenty-one strains from backyard farms and live bird markets formed three new clusters within lineage 5, tentatively named 5f, 5g and 5h. All of these strains were predicted to be virulent based on their F protein cleavage site sequence. Minimal genetic distances between new and previously established sublineages ranged from 9.4 to 15.9%, and minimal distances between the new sublineages were 11.5 to 17.3%. Their high genetic diversity and their presence in three different Sub-Saharan countries suggest that these new sublineages represent the NDV variants indigenous to West Africa.