Hard tissue formation in subcutaneously transplanted rat dental pulp
Hard tissue formation in subcutaneously transplanted rat dental pulp
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DOI：
10.1177/154405910708600515
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发表时间：
2007-05-01
影响因子：
7.6
通讯作者：
Ozawa, H.
中科院分区：
文献类型：
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作者：
Hosoya, A.;Nakamura, H.;Ozawa, H.
虽然牙髓似乎能够形成并不总是类似牙本质的矿化基质，但硬组织的确切特征及其诱导机制仍不清楚。因此，我们评估了将牙髓移植到皮下组织中所诱导的硬组织。移植后7天，牙髓内周缘形成初始硬组织。14天后，这些硬组织向内扩张。矿化基质骨钙素、骨桥蛋白和骨涎蛋白免疫阳性，而牙本质涎蛋白阴性。将GFP标记的牙髓移植到野生型大鼠身上，结果显示这些形成细胞来自移植。扫描电子显微镜观察发现，在钙化初期，坏死细胞和基质囊泡内有磷灰石晶体。这些结果表明牙髓细胞具有形成骨样或牙骨质样基质的能力。基质的钙化可能发生在坏死细胞和基质小泡中，随后是胶原性钙化。
While dental pulp appears to be able to form mineralized matrices that do not always resemble dentin, the precise characteristics of the hard tissue and the mechanism of its induction remain unknown. Therefore, we evaluated hard tissue induced by transplantation of pulp into subcutaneous tissue. Seven days after transplantation, initial hard tissue was formed at the inner periphery of the pulp. After 14 days, this hard tissue expanded inwardly. Mineralized matrix was immunopositive for osteocalcin, osteopontin, and bone sialoprotein, but negative for dentin sialoprotein. Transplantation of GFP-labeled pulp into wild-type rats showed these formative cells to have been derived from the transplant. TEM observation revealed apatite crystals within necrotic cells and matrix vesicles at the initial stage of calcification. These results indicate that pulp cells possess the ability to form a bone- or cementum-like matrix. Calcification of the matrix may occur in necrotic cells and matrix vesicles, followed by collagenous calcification.