The bright frontiers of microbial metabolic optogenetics
The bright frontiers of microbial metabolic optogenetics
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微生物代谢光遗传学的光明前沿
DOI：
10.1016/j.cbpa.2022.102207
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发表时间：
2022
影响因子：
7.8
通讯作者：
Avalos, José L.
中科院分区：
文献类型：
--
作者：
Wegner, Scott A.;Barocio-Galindo, Rachel M.;Avalos, José L.
近年来，光遗传学领域的光响应系统已被应用于代谢工程的几个领域，并取得了显着的成功。通过利用光对宿主内源性过程的高度可调谐性、可逆性和正交性，光遗传学系统已经实现了对用于化学生产、代谢通量分析和共培养物中的群体组成的微生物发酵的前所未有的动态控制。在这篇文章中，我们分享了我们对代谢光遗传学这一新领域的现状的看法，我们认为它将继续在越来越多的新方向上影响代谢工程，并有可能挑战现有的代谢途径和菌株优化以及生物反应器操作的范式。
In recent years, light-responsive systems from the field of optogenetics have been applied to several areas of metabolic engineering with remarkable success. By taking advantage of light's high tunability, reversibility, and orthogonality to host endogenous processes, optogenetic systems have enabled unprecedented dynamical controls of microbial fermentations for chemical production, metabolic flux analysis, and population compositions in co-cultures. In this article, we share our opinions on the current state of this new field of metabolic optogenetics.We make the case that it will continue to impact metabolic engineering in increasingly new directions, with the potential to challenge existing paradigms for metabolic pathway and strain optimization as well as bioreactor operation.