Peptide-specific T cell clonal expansion in vivo following immunization in the eye, an immune-privileged site.
Peptide-specific T cell clonal expansion in vivo following immunization in the eye, an immune-privileged site.
复制标题
DOI：
10.4049/jimmunol.157.6.2262
复制
发表时间：
1996-09
影响因子：
4.4
通讯作者：
R. Egan;C. Yorkey;R. Black;Wai Khan Loh;J. L. Stevens;J. Woodward
中科院分区：
文献类型：
--
作者：
R. Egan;C. Yorkey;R. Black;Wai Khan Loh;J. L. Stevens;J. Woodward
为了观察对引入眼中的Ag的初级抗原特异性T细胞应答，我们使用了过继转移系统，其中将来自TCR转基因小鼠的有限数量的OVA肽（323-339）特异性T细胞转移到未照射的同基因受体中，然后通过染色进行FACS分析或用克隆型mAb KJ 1 -26进行免疫组织化学来体内追踪。后房注射Ag后，KJ 1 -26+细胞在3天内主要聚集在引流的下颌下淋巴结（LN）中。虽然数量减少，但到第6天，这些细胞主要位于副皮质区，并且能够响应Ag刺激而增殖和分泌IL-2。与此相反，静脉注射Ag后，KJ 1 -26+细胞在LN的副皮质区积累到相当的程度，但不增殖或分泌IL-2。如果在注射Ag后5 h内摘除眼睛，则第3天KJ 1 -26+细胞在颌下LN中的积聚受到抑制。在脾脏中，第3天在动脉周围淋巴鞘中观察到KJ 1 -26+细胞灶;在其他形式的免疫接种后未观察到相同程度的KJ 1 -26+细胞灶。这些结果表明，下颌下LN是早期克隆性扩增的抗原特异性T细胞眼内Ag管理后的主要网站，这些细胞表现出一致的免疫，而不是耐受性的变化。
To visualize the primary antigen-specific T cell response to Ag introduced into the eye, we have used an adoptive transfer system in which a limiting number of OVA peptide (323-339)-specific T cells from a TCR-transgenic mouse were transferred into nonirradiated, syngeneic recipients and then tracked in vivo by staining for FACS analysis or immunohistochemistry with the clonotypic mAb KJ1-26. Following posterior chamber injection of Ag, KJ1-26+ cells accumulated primarily in the draining, submandibular lymph node (LN) within 3 days. Although reduced in number, by day 6 these cells were primarily in the paracortical regions and were able to proliferate and secrete IL-2 in response to Ag stimulation. In contrast, following i.v. injection of Ag, the KJ1-26+ cells accumulated in the paracortical regions of the LN to a comparable degree, but did not proliferate or secrete IL-2. The day 3 accumulation of KJ1-26+ cells in the submandibular LN was inhibited if the eye was removed within 5 h after injection of Ag. In the spleen, foci of KJ1-26+ cells were observed in the periarteriolar lymphoid sheaths at day 3; these were not observed to the same degree following other forms of immunization. These results demonstrate that the submandibular LN is the primary site for early clonal expansion of antigen-specific T cells following intraocular Ag administration and that these cells show changes consistent with immunity rather than tolerance.