Central oxytocin is involved in restoring impaired gastric motility following chronic repeated stress in mice
Central oxytocin is involved in restoring impaired gastric motility following chronic repeated stress in mice
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DOI：
10.1152/ajpregu.00328.2009
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Takahashi, Toku
中科院分区：
文献类型：
--
作者：
Babygirija, Reji;Zheng, Jun;Takahashi, Toku
王晓明，王晓明，王晓明，等。中央区催产素参与慢性反复应激小鼠胃动力损伤的恢复。[J] .中国生物医学工程学报，2016,31(2):557 - 557。首次发表于2009年11月4日；doi: 10.1152 / ajpregu.00328.2009。-持续生活压力（慢性压力）的积累常引起胃部症状。胃症状的发展可能取决于人类如何适应日常生活中的应激事件。虽然急性应激延迟了啮齿动物的胃排空并改变了上消化道运动，但慢性应激对胃运动的影响及其适应机制尚不清楚。中枢催产素已被证明具有抗压力作用。我们研究中枢催产素是否参与慢性重复应激后的适应机制。小鼠分别承受急性和慢性应激（连续5天重复应激），比较急性和慢性重复应激对胃实体排空和餐后胃运动的影响。研究了急性和慢性反复应激后下丘脑中催产素和CRF mRNA的表达。急性应激期胃排空延迟（43.1 +/- 7.8%,n = 6, P < 0.05）在慢性重复应激后完全恢复到正常水平（72.1 +/- 2.4%,n = 6）。急性应激引起的胃运动障碍在慢性重复应激后也能恢复。脑室内注射催产素（0.1和0.5 μ g）可恢复急性应激引起的胃排空和胃运动功能受损。脑室内注射催产素拮抗剂可拮抗慢性反复应激后胃排空和胃动力的恢复。慢性重复应激后下丘脑视上核（SON）和室旁核（PVN）的催产素mRNA表达显著升高。相比之下，急性应激下SON和PVN中CRF mRNA表达的增加在慢性重复应激后显著降低。本研究提示，中枢催产素表达上调参与了小鼠慢性反复应激后的适应机制。
Babygirija R, Zheng J, Ludwig K, Takahashi T. Central oxytocin is involved in restoring impaired gastric motility following chronic repeated stress in mice. Am J Physiol Regul Integr Comp Physiol 298: R157-R165, 2010. First published November 4, 2009; doi:10.1152/ajpregu.00328.2009.-Accumulation of continuous life stress (chronic stress) often causes gastric symptoms. The development of gastric symptoms may depend on how humans adapt to the stressful events in their daily lives. Although acute stress delays gastric emptying and alters upper gastrointestinal motility in rodents, the effects of chronic stress on gastric motility and its adaptation mechanism remains unclear. Central oxytocin has been shown to have antistress effects. We studied whether central oxytocin is involved in mediating the adaptation mechanism following chronic repeated stress. Mice were loaded with acute and chronic stress (repeated stress for five consecutive days), and solid gastric emptying and postprandial gastric motility were compared between acute and chronic repeated stress. Expression of oxytocin and CRF mRNA in the hypothalamus was studied following acute and chronic repeated stress. Delayed gastric emptying during acute stress (43.1 +/- 7.8%; n = 6, P < 0.05) was completely restored to normal levels (72.1 +/- 2.4%; n = 6) following chronic repeated stress. Impaired gastric motility induced by acute stress was also restored following chronic repeated stress. Intracerebroventricular injection of oxytocin (0.1 and 0.5 mu g) restored the impaired gastric emptying and motility induced by acute stress. The restored gastric emptying and motility following chronic repeated stress were antagonized by intracerebroventricular injection of oxytocin antagonists. Oxytocin mRNA expression in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the hypothalamus was significantly increased following chronic repeated stress. In contrast, increased CRF mRNA expression in the SON and PVN in response to acute stress was significantly reduced following chronic repeated stress. Our study suggests the novel finding that the upregulation of central oxytocin expression is involved in mediating the adaptation mechanism following chronic repeated stress in mice.